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Species: HPV
Genetic Insert: HPV 18 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37886 Copy
Species: HPV
Genetic Insert: HPV 8 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: The 8E7 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)
Proper citation: RRID:Addgene_37920 Copy
Species: HPV
Genetic Insert: HPV 16 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37881 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BDLF3.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37929 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22483942
Comments: This construct is resistant to shRNA #3.
EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.
Proper citation: RRID:Addgene_37925 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37928 Copy
Species: HPV
Genetic Insert: HPV 11 E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37873 Copy
Genetic Insert: HPV 16 E5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37874 Copy
Species: HPV
Genetic Insert: HPV 8 E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37919 Copy
Species: HPV
Genetic Insert: HPV 6b E5b
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37914 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Operon: cfp upstream of yfp
Chromosomal integration at grtAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T710 YFP::Asp
Proper citation: RRID:Addgene_37987 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Operon: yfp upstream of cfp
Chromosomal integration at galM and/or galK: KanRnoFRT, pLlacO-1:T710 YFP::T710 CFP::Asp
Proper citation: RRID:Addgene_37988 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-coregulated, Non-clustered
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1
Chromosomal integration at galM and/or galK: T1::pLtetO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37983 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-coregulated, codirectional: cfp upstream of yfp
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1::pLtetO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37985 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Strain description: CsrA system knockout
Chromosomal changes: ΔCsrA, ΔCsrB, ΔCsrC, ΔglgCAP, ΔpgaABCD, +LacIq at galK
Proper citation: RRID:Addgene_37980 Copy
Species: Strongylocentrotus purpuratus
Genetic Insert: ABCB1a
Vector Backbone Description: Backbone Size:4851; Vector Backbone:pCS2+; Vector Types:Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23124201
Proper citation: RRID:Addgene_38036 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-clustered
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1
Chromosomal integration at galM and/or galK: T1::pLlacO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37982 Copy
Species: Homo sapiens
Genetic Insert: Nucleoporin 98
Vector Backbone Description: Backbone Size:2968; Vector Backbone:pPEP-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21685911
Comments: see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.
Proper citation: RRID:Addgene_38037 Copy
Species: Homo sapiens
Genetic Insert: shREV3-5
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:4354; Vector Backbone:pSUPERIOR.puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Proper citation: RRID:Addgene_38030 Copy
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