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Species: Synthetic
Genetic Insert: luxB
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2876; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188306 Copy
Species: Synthetic
Genetic Insert: luxE
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2876; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188309 Copy
Species: Synthetic
Genetic Insert: fre
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2918; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188310 Copy
Genetic Insert: 3X GFP
Vector Backbone Description: Backbone Size:4463; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35545715
Proper citation: RRID:Addgene_191206 Copy
Species: Synthetic
Genetic Insert: GFP-Luz fusion
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2912; Vector Backbone:pCI-T7Max-UTR1-NTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188314 Copy
Species: Synthetic
Genetic Insert: Luz-GFP fusion
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2867; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188315 Copy
Vector Backbone Description: Backbone Marker:TRC; Backbone Size:8952; Vector Backbone:PLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39167658
Comments: Please visit https://doi.org/10.1101/2024.05.19.594864 for bioRxiv preprint.
Proper citation: RRID:Addgene_191566 Copy
Species: Synthetic
Genetic Insert: P2A
Vector Backbone Description: Backbone Marker:John Deuber; Backbone Size:1666; Vector Backbone:pYTK001; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_190912 Copy
Genetic Insert: UNG-rAPOBEC1(R33A)-zCas9(D10A)
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173997 Copy
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:4466; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191208 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-UNG
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173998 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-rXRCC1
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173999 Copy
Species: Synthetic
Genetic Insert: O2A
Vector Backbone Description: Backbone Marker:John Deuber; Backbone Size:1668; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_190917 Copy
Species: Synthetic
Genetic Insert: HiBiT-GFP fusion
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2873; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188317 Copy
Species: Homo sapiens
Genetic Insert: cERb447
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36163385
Proper citation: RRID:Addgene_188257 Copy
Species: Homo sapiens
Genetic Insert: RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6790; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31105010
Proper citation: RRID:Addgene_187282 Copy
Species: Homo sapiens
Genetic Insert: cLYN268
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36163385
Proper citation: RRID:Addgene_188259 Copy
Vector Backbone Description: Vector Backbone:pYPQ134A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_173930 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Tsc1
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33608386
Proper citation: RRID:Addgene_173658 Copy
Species: Homo sapiens
Genetic Insert: MAP3K14
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189903 Copy
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