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Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113182 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113188 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113187 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113176 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113181 Copy
Species: E. coli
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113633 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113762 Copy
Species: E. coli
Genetic Insert: clbO
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114153 Copy
Species: E. coli
Genetic Insert: clbK
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114152 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114157 Copy
Species: E. coli
Genetic Insert: clbB
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114155 Copy
Species: E. coli
Genetic Insert: ClbD
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114146 Copy
Species: E. coli
Genetic Insert: ClbF
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114148 Copy
Species: E. coli
Genetic Insert: ClbE
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114147 Copy
Species: E. coli
Genetic Insert: OsmC
Vector Backbone Description: Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15103136
Comments: Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11436 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105839 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105844 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105845 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105843 Copy
Species: E. coli
Genetic Insert: Maltose-binding protein (MalE)
Vector Backbone Description: Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30600549
Comments: Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7
Proper citation: RRID:Addgene_107422 Copy
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