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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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pRW004-SM-destination-proUBQ10-Cas9 Resource Report Resource Website |
RRID:Addgene_104438 | Spectinomycin | PMID:30083222 | There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency. | Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:44 | 0 | |||
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pUG-UBC19 Resource Report Resource Website |
RRID:Addgene_104684 | AtUBC19 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC15 Resource Report Resource Website |
RRID:Addgene_104680 | AtUBC15 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC16 Resource Report Resource Website |
RRID:Addgene_104681 | AtUBC16 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC23 Resource Report Resource Website |
RRID:Addgene_104688 | AtUBC23 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC22 Resource Report Resource Website |
RRID:Addgene_104687 | AtUBC22 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-UBC31 Resource Report Resource Website |
RRID:Addgene_104696 | AtUBC31 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-UBC25 Resource Report Resource Website |
RRID:Addgene_104690 | AtUBC25 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC34 Resource Report Resource Website |
RRID:Addgene_104699 | AtUBC34 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-UBC33 Resource Report Resource Website |
RRID:Addgene_104698 | AtUBC33 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG-138 Resource Report Resource Website |
RRID:Addgene_104706 | MMZ4/UEV1D | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-248 Resource Report Resource Website |
RRID:Addgene_104709 | PUB22 C13A | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | C13A, BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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pUG41780 Resource Report Resource Website |
RRID:Addgene_104662 | EL#4 | Synthetic | Spectinomycin | PMID:29194629 | Level 2 end linker | Backbone Marker:self-made; Backbone Size:3298; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG41766 Resource Report Resource Website |
RRID:Addgene_104661 | EL#3 | Synthetic | Spectinomycin | PMID:29194629 | Level 2 end linker | Backbone Marker:self-made; Backbone Size:3298; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG41744 Resource Report Resource Website |
RRID:Addgene_104660 | EL#2 | Synthetic | Spectinomycin | PMID:29194629 | Level 2 end linker | Backbone Marker:self-made; Backbone Size:3298; Vector Backbone:pUC19-derived; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-135 Resource Report Resource Website |
RRID:Addgene_104703 | MMZ1/UEV1A | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-136 Resource Report Resource Website |
RRID:Addgene_104704 | MMZ2/UEV1B | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-UBC1 Resource Report Resource Website |
RRID:Addgene_104666 | AtUBC1 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-UBC36 Resource Report Resource Website |
RRID:Addgene_104701 | AtUBC36 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-09-26 02:18:46 | 0 | |
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pUG-350 Resource Report Resource Website |
RRID:Addgene_104718 | Fen | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | Addgene | 2026-09-26 02:18:46 | 0 |
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