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Genetic Insert: E. coli K-12 MG1655_rLP6(ygcE-ygcF)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29388765
Comments: Genotype: F- lambda- ilvG- rfb-50 rph-1 lox71 mCherry,ChlorR loxm2/66
Proper citation: RRID:Addgene_110246 Copy
Species: Synthetic
Genetic Insert: T7 promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110574 Copy
Species: Synthetic
Genetic Insert: CP25 Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110575 Copy
Species: Synthetic
Genetic Insert: CP32 Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110578 Copy
Species: Synthetic
Genetic Insert: CP12b Promoter with RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110577 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Novagen/Agilent; Backbone Size:2412; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: pIM2432
Proper citation: RRID:Addgene_110547 Copy
Species: Synthetic
Genetic Insert: rpoC
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110592 Copy
Species: Synthetic
Genetic Insert: Lac promoter with lac operator and RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110582 Copy
Vector Backbone Description: Backbone Size:2979; Vector Backbone:pUCDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110742 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30478458
Comments: The three new “Marionette” strains contain the Marionette cluster, which expresses 12 inducible transcription factors, from the genome. Plasmids pAJM.711, pAJM.712, pAJM.713, pAJM.714, pAJM.715, pAJM.717, pAJM.716, pAJM.718, pAJM.719, pAJM.1459, pAJM.721 are reporters for those 12 transcription factors. For examples: TetR is being expressed from the genome of the Marionette strains, and pAJM.717 has a promoter that is repressed by TetR (PTet*) driving YFP. pAJM.717 should not produce YFP, until the cell is exposed to anhydrotetracycline (aTc). Other plasmids from our laboratory encode both the reporter and the transcription factor, and should be used in a regular (non-Marionette) strain. For example: pAJM.011 expresses TetR and contains a PTet*-YFP reporter module.
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108254 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2230; Vector Backbone:pHSG398; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108203 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBC SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11771662
Proper citation: RRID:Addgene_108311 Copy
Species: Synthetic
Genetic Insert: attB-eGFP-4xGly-TEV-Flag-6xHis
Vector Backbone Description: Backbone Marker:Kenan Murphy; Backbone Size:3159; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating EGFP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108434 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1-UGI-LVA
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29403014
Proper citation: RRID:Addgene_108551 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pBC SK; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109061 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109058 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109059 Copy
Species: Synthetic
Genetic Insert: attB-Flag-DAS+4
Vector Backbone Description: Backbone Size:3139; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating Flag-DAS4+ fusions to mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109284 Copy
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