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Species: E. Coli
Genetic Insert: LacI-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60756 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: This plasmid contains I100V, K245R and F258L mutations in the ADE2 gene that do not impact selection or plasmid function.
Proper citation: RRID:Addgene_60932 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Proper citation: RRID:Addgene_60930 Copy
Species: E. coli
Genetic Insert: SCRIBE_kanR(ON)
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61450 Copy
Species: E. coli
Genetic Insert: Beta recombinase
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZA11; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61446 Copy
Species: E. coli
Genetic Insert: nptII gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter).
NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp).
Proper citation: RRID:Addgene_61767 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61972 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61973 Copy
Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61974 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4541; Vector Backbone:pQE-80L-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25691744
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_62598 Copy
Species: E. coli
Genetic Insert: HPH
Vector Backbone Description: Backbone Marker:Messing Lab; Vector Backbone:pFF19, Addgene plasmid #49777; Vector Types:plant expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1369289
Comments: polylinker: SalI, XbaI, BamHI, SmaI, KpnI, SstI/SacI, NruI
Proper citation: RRID:Addgene_49783 Copy
Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
Species: E. coli
Genetic Insert: Relevant genotype: supE, thi, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Proper citation: RRID:Addgene_50349 Copy
Species: E. coli
Genetic Insert: Relevant genotype: ara, Δ(lac-proAB), rspL(+strA), ϕ80, lacZΔM15
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Proper citation: RRID:Addgene_50348 Copy
Species: E. coli
Genetic Insert: Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed)
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA
This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.
A mutS+ version of this strain will be deposited with addgene soon.
Genome sequence deposited at NCBI (accession # CP006698)
Proper citation: RRID:Addgene_48998 Copy
Species: E. coli
Genetic Insert: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA
This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).
Proper citation: RRID:Addgene_49018 Copy
Species: E. coli
Genetic Insert: CsrA
Vector Backbone Description: Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23878244
Proper citation: RRID:Addgene_37571 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfpAaV
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37760 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfpAaV
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37759 Copy
Species: E. coli
Genetic Insert: AraC
Vector Backbone Description: Vector Backbone:pHG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34369028
Proper citation: RRID:Addgene_170114 Copy
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