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Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234574 Copy
Species: Homo sapiens
Genetic Insert: SUZ12
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234575 Copy
Species: Homo sapiens
Genetic Insert: RBBP4
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234577 Copy
Species: Homo sapiens
Genetic Insert: AEBP2 [Isoform 3]
Vector Backbone Description: Vector Backbone:pMB.001; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Proper citation: RRID:Addgene_234578 Copy
Genetic Insert: dCas9, sgRNA expression cassette, sfGFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression, pir dependent origin of replication; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38126769
Proper citation: RRID:Addgene_208888 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies.
Proper citation: RRID:Addgene_201003 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5135; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies.
Proper citation: RRID:Addgene_200991 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer
Proper citation: RRID:Addgene_228668 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer with PmeI cleavage site and biGBac linker sequence A&B
Proper citation: RRID:Addgene_228675 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer with PmeI cleavage site and biGBac linker sequence B&C
Proper citation: RRID:Addgene_228676 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer with PmeI cleavage site and biGBac linker sequence D&E
Proper citation: RRID:Addgene_228678 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254905 Copy
Species: Homo sapiens
Genetic Insert: Dipeptidyl peptidase 9
Vector Backbone Description: Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39552560
Proper citation: RRID:Addgene_254906 Copy
Species: Pseudomonas aeruginosa PA14
Genetic Insert: Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14
Vector Backbone Description: Backbone Marker:Herbert Schweizer (Addgene #63121); Backbone Size:4500; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:40904105
Comments: Synthetic Type IF CRISPR-Cas system based on the sequence from Pseudomonas aeruginosa PA14 with a minimised version of the CRISPR 2 array containing a non-targeting spacer with BbsI restriction sites for cloning. Native promoter regions were cloned as the intergenic region between the CRISPR components and upstream genes. MluI, NsiI, NotI, NcoI, AscI restriction sites were added to make the system modular.
Proper citation: RRID:Addgene_246943 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205022 Copy
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110739 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: GFP
Proper citation: RRID:Addgene_228667 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer with PmeI cleavage site and biGBac linker sequence E&F
Proper citation: RRID:Addgene_228679 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:39814169
Comments: Spacer with PmeI cleavage site and biGBac linker sequence C&D
Proper citation: RRID:Addgene_228677 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:38715147
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201004 Copy
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