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Species: Homo sapiens
Genetic Insert: TJP1
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4670; Vector Backbone:pET14b_TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22030391
Proper citation: RRID:Addgene_59731 Copy
Species: Homo sapiens
Genetic Insert: CBX1 Chromo
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Comments: The discrepancies found in the QC sequence do not have any functional consequence.
Proper citation: RRID:Addgene_59697 Copy
Species: T. brucei Lister 427
Genetic Insert: VSG221 RNAi fragment
Vector Backbone Description: Vector Backbone:pBluescriptII; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15937117
Comments: Please note that Addgene QC did not confirm the RNAi fragment, but the linear size of the digested vector give the predicted result.
Proper citation: RRID:Addgene_59730 Copy
Species: Homo sapiens
Genetic Insert: KDM4A double Tudor
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Comments: The discrepancies found in the QC sequence do not have any functional consequence.
Proper citation: RRID:Addgene_59699 Copy
Species: Homo sapiens
Genetic Insert: zonula occludens-1 PDZ2
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4670; Vector Backbone:pET14b_TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17928286
Proper citation: RRID:Addgene_59734 Copy
Species: Drosophila melanogaster
Genetic Insert: Or98a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10089887
Comments: source: Genomic DNA
Proper citation: RRID:Addgene_59692 Copy
Species: Homo sapiens
Genetic Insert: MPHOSPH8 Chromo
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Proper citation: RRID:Addgene_59694 Copy
Species: Mus musculus
Genetic Insert: DNMT3A PWWP
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Comments: Note: P144 is deleted, which has no functional consequences on the plasmid.
Proper citation: RRID:Addgene_59696 Copy
Species: Homo sapiens
Genetic Insert: CBX7 Chromo
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Proper citation: RRID:Addgene_59695 Copy
Species: Homo sapiens
Genetic Insert: zonula occludens-1
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4670; Vector Backbone:pET14b_TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_59739 Copy
Species: Homo sapiens
Genetic Insert: PML isoform IV
Vector Backbone Description: Backbone Size:4713; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24882209
Proper citation: RRID:Addgene_59742 Copy
Vector Backbone Description: Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_59744 Copy
Species: Mus musculus
Genetic Insert: ELYS-emerin-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59745 Copy
Species: Mus musculus
Genetic Insert: LBR-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59748 Copy
Species: Mus musculus
Genetic Insert: emerin-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59747 Copy
Species: Mus musculus
Genetic Insert: LBR-mRFP1
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59749 Copy
Genetic Insert: attL1-em7-kanR-attL2-pA
Vector Backbone Description: Vector Backbone:pBluescript SKII-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924516
Proper citation: RRID:Addgene_59764 Copy
Species: Synthetic
Genetic Insert: CAS9
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23997119
Comments: The eGFP ORF was inserted into C-terminal of CAS9 ORF of
pCAG-T3-hCAS-pA (Addgene Plasmid #48625).
For the in vitro synthesis of CAS9 mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9eGFP-expression vector.
Proper citation: RRID:Addgene_59766 Copy
Species: Homo sapiens
Genetic Insert: RBFOX2
Vector Backbone Description: Backbone Size:13918; Vector Backbone:pSLIK Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637117
Proper citation: RRID:Addgene_59769 Copy
Species: Synthetic
Genetic Insert: mRuby2_smFP_FLAG
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59760 Copy
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