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Genetic Insert: QF-T7-TetO-TetR
Vector Backbone Description: Backbone Size:3062; Vector Backbone:T7-LacO-QF_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171661 Copy
Species: Homo sapiens
Genetic Insert: myc-PDGFRA-R914W
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10375; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, EGFP-PEST_Reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35147171
Proper citation: RRID:Addgene_172596 Copy
Genetic Insert: T7-LacO-TetR-T7 Stop
Vector Backbone Description: Backbone Size:3192; Vector Backbone:T7-TetO-QF_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171659 Copy
Genetic Insert: T7-5-QUAS
Vector Backbone Description: Backbone Size:2761; Vector Backbone:QUAS-0-T7-GFP_ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171656 Copy
Genetic Insert: FRO6p::GUSPlus::tOCS
Vector Backbone Description: Vector Backbone:pYI001; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:36216814
Comments: Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint.
Proper citation: RRID:Addgene_170840 Copy
Genetic Insert: QUAS-15-T7
Vector Backbone Description: Backbone Size:2761; Vector Backbone:QUAS-0-T7-GFP_ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171655 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:6497; Vector Backbone:pRC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_188115 Copy
Genetic Insert: QUAS-5-T7
Vector Backbone Description: Vector Backbone:AmpColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171653 Copy
Species: Homo sapiens
Genetic Insert: SMASh-SpdCas9-tagRFPt-P2A-tagBFP
Vector Backbone Description: Backbone Size:11406; Vector Backbone:pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37270532
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.05.511019v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_187949 Copy
Species: Homo sapiens
Genetic Insert: ER50-SpdCas9-tagRFPt-P2A-tagBFP
Vector Backbone Description: Backbone Size:11406; Vector Backbone:pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37270532
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.05.511019v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_187948 Copy
Species: Synthetic
Genetic Insert: SpdCas9-tagRFPt-P2A-tagBFP
Vector Backbone Description: Backbone Size:5702; Vector Backbone:pSLPB2; Vector Types:Mammalian Expression, Synthetic Biology, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37270532
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.05.511019v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_187945 Copy
Genetic Insert: QF-T7 Stop
Vector Backbone Description: Backbone Size:2101; Vector Backbone:T7-LacO-Kan_p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171651 Copy
Genetic Insert: T7-0-QUAS
Vector Backbone Description: Backbone Size:2761; Vector Backbone:QUAS-T7-0-GFP_ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226549
Proper citation: RRID:Addgene_171650 Copy
Species: Homo sapiens
Genetic Insert: WEE2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189895 Copy
Genetic Insert: tTA
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191210 Copy
Species: S. purpuratus
Genetic Insert: AGS-dGoLoco1
Vector Backbone Description: Vector Backbone:PSP64; Vector Types:In vitro transcription (mRNA synthesis); Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439829
Proper citation: RRID:Addgene_185577 Copy
Species: Homo sapiens
Genetic Insert: DCLK3
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189891 Copy
Species: Synthetic
Genetic Insert: alr4766
Vector Backbone Description: Backbone Size:5303; Vector Backbone:pSEVA251; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_186707 Copy
Species: Rosa multiflora
Genetic Insert: RmMYB108
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34386027
Proper citation: RRID:Addgene_173183 Copy
Species: Rosa multiflora
Genetic Insert: RmMYB108
Vector Backbone Description: Vector Backbone:pCAMBIA1301; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34386027
Proper citation: RRID:Addgene_173180 Copy
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