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Species: Synthetic
Genetic Insert: hSpCas9
Vector Backbone Description: Vector Backbone:px458 (Plasmid #48138); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38458201
Proper citation: RRID:Addgene_215318 Copy
Species: Homo sapiens
Genetic Insert: WDR20
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37798301
Proper citation: RRID:Addgene_217618 Copy
Species: Mus musculus
Genetic Insert: GfaABC1D-Crym-BioID2-BioID2
Vector Backbone Description: Backbone Size:3037; Vector Backbone:PZac2.1; Vector Types:Mouse Targeting, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38418885
Proper citation: RRID:Addgene_200070 Copy
Species: Glycine max (soybean)
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pLV UBC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: To clone something N-terminal to APEX2, digest with NheI. Add the following overhangs to forward and reverse primers of the insert: F: 5'-TCCATAGAAGACACCGACGCCACCATG[inset 5' overlap]-3', R: 5'-TCCATAGAAGACACCGACGCCACCATG[Insert 3' overlap reverse complement]-3'. On the 3' side, include a small linker, otherwise there is very little spacer between the insert and APEX2. Additional data, code, and other details related to this work (Hollingsworth et al. 2024) are available at https://harperlab.pubpub.org/pub/nlrp3/.
Proper citation: RRID:Addgene_214983 Copy
Species: Homo sapiens
Genetic Insert: hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Vector Backbone Description: Vector Backbone:pTwist-Amp; Vector Types:HDR template; Bacterial Resistance:Ampicillin
Comments: Use either of the following guides: GTCTGGAGTTACAGCGTCGGGGG or CCGCTCATTGAGTGCCTTCAGGG (in both cases, the PAM is the final GGG) . Additional data, code, and other details related to this work (Hollingsworth et al. 2024) are available at https://harperlab.pubpub.org/pub/nlrp3/.
Proper citation: RRID:Addgene_214998 Copy
Species: Homo sapiens
Genetic Insert: hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Vector Backbone Description: Vector Backbone:pTwist-Amp; Vector Types:HDR template; Bacterial Resistance:Ampicillin
Comments: Use either of the following guides: GTCTGGAGTTACAGCGTCGGGGG or CCGCTCATTGAGTGCCTTCAGGG (in both cases, the PAM is the final GGG) . Additional data, code, and other details related to this work (Hollingsworth et al. 2024) are available at https://harperlab.pubpub.org/pub/nlrp3/.
Proper citation: RRID:Addgene_214999 Copy
Species: Mus musculus
Genetic Insert: EEA1
Vector Backbone Description: Vector Backbone:pUC-GW-Amp; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Target site is: CCGGCCAAACCATGTTTCGAAGG (AGG is the PAM). Additional data, code, and other details related to this work (Hollingsworth et al. 2024) are available at https://harperlab.pubpub.org/pub/nlrp3/.
Proper citation: RRID:Addgene_214990 Copy
Species: Homo sapiens
Genetic Insert: Ras-LOCKR-S
Vector Backbone Description: Backbone Marker:Genscript; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38273065
Proper citation: RRID:Addgene_216496 Copy
Species: Synthetic
Genetic Insert: LacI**-eGFP
Vector Backbone Description: Backbone Size:2888; Vector Backbone:PB-empty; Vector Types:Mammalian Expression, Mouse Targeting, Piggybase integration (Transposon); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36471076
Proper citation: RRID:Addgene_213021 Copy
Species: Synthetic
Genetic Insert: SlugABE-NNG
Vector Backbone Description: Vector Backbone:AAV2 ITR vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38052959
Proper citation: RRID:Addgene_214356 Copy
Genetic Insert: Cas9-2A-puro
Vector Backbone Description: Vector Backbone:ModRNAc1; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38579711
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.09.07.556774v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216390 Copy
Species: Homo sapiens
Genetic Insert: CCND3
Vector Backbone Description: Vector Backbone:mAzamiGreen (Plasmid #54797); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38458201
Proper citation: RRID:Addgene_215061 Copy
Species: Homo sapiens
Genetic Insert: CCND1
Vector Backbone Description: Vector Backbone:mAzamiGreen (Plasmid #54797); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38458201
Proper citation: RRID:Addgene_215060 Copy
Species: Homo sapiens
Genetic Insert: CACTGAATACCAGCGCCTAG
Vector Backbone Description: Vector Backbone:pX330 Addgene #42230; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37115157
Proper citation: RRID:Addgene_207557 Copy
Genetic Insert: (mito).cpSFGFP.HaloTag
Vector Backbone Description: Vector Backbone:pAAV.CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38748581
Comments: Please see bioRxiv preprint at https://doi.org/10.1101/2023.08.24.554624
Proper citation: RRID:Addgene_214925 Copy
Genetic Insert: Cas9-2A-p53DD
Vector Backbone Description: Vector Backbone:ModRNAc1; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38579711
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.09.07.556774v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216389 Copy
Genetic Insert: VP64dCas9VP64-2A-MPH
Vector Backbone Description: Vector Backbone:ModRNAc1; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38579711
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.09.07.556774v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216388 Copy
Species: Homo sapiens
Genetic Insert: GLI3
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_000168,ENST00000395925 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_143655 Copy
Species: Dreissena polymorpha
Genetic Insert: DpFnuc
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37756409
Proper citation: RRID:Addgene_217492 Copy
Species: Homo sapiens
Genetic Insert: phospholipase B domain containing 2
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38447580
Proper citation: RRID:Addgene_213607 Copy
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