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pCI-MEG3c
 
Resource Report
Resource Website 		RRID:Addgene_59422 MEG3c Homo sapiens Ampicillin PMID:17569660 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 0
pMKO.1-puro/hFOXH1 shRNA1
 
Resource Report
Resource Website 		RRID:Addgene_59303 hFOXH1 shRNA Homo sapiens Ampicillin PMID:24759836 Backbone Marker:Addgene Plasmid #8452; Backbone Size:6700; Vector Backbone:pMKO.1-puro; Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:17:22 0
pLenti CMV-IFP2.0 T2A HO1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59427 IFP2.0T2AHO1 IRES EGFP Ampicillin PMID:24832154 Backbone Marker:addgene; Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST (670-1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 3
pT2SC
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59382 TT-ISceI-cat Synthetic Chloramphenicol and Ampicillin PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:23 1
pT2ST
 
Resource Report
Resource Website 		RRID:Addgene_59384 TT-ISceI-dfrA Synthetic Ampicillin and Trimethoprim PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™ (Epicentre). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Trimethoprim 2026-08-15 01:17:23 0
pT2SK
 
Resource Report
Resource Website 		RRID:Addgene_59383 TT-ISceI-kan Synthetic Ampicillin and Kanamycin PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The kanamycin resistance gene was taken from pACYC177. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-15 01:17:23 0
pSLTS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59386 Ampicillin PMID:25255806 We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Herring and Blattner; Backbone Size:7742; Vector Backbone:pKDTS; Vector Types:Bacterial Expression, Genome Engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 2
pT2SCb
 
Resource Report
Resource Website 		RRID:Addgene_59385 TT-ISceI-cat2 Synthetic Chloramphenicol and Ampicillin PMID:25255806 iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin The 3’-end of the chloramphenicol resistance gene was modified to 5’-AGGAGGTGCATAA to create a sequence resembling the consensus bacterial ribosomal binding site (5’-AGGAGGTAAATAA). 2026-08-15 01:17:23 0
pFRT-TODestFLAGHA_RBPMS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59388 RBPMS Homo sapiens Ampicillin PMID:24860013 Backbone Marker: Life Technologies, Modified by Tuschl Lab ; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 2
pCI-MEG3b
 
Resource Report
Resource Website 		RRID:Addgene_59421 MEG3b Homo sapiens Ampicillin PMID:17569660 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 0
pCI-MEG3a
 
Resource Report
Resource Website 		RRID:Addgene_59420 MEG3a Homo sapiens Ampicillin PMID:17569660 Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 0
pet28a_RBPMS_FL
 
Resource Report
Resource Website 		RRID:Addgene_59391 RBPMS Homo sapiens Kanamycin PMID:24860013 Backbone Marker:Novagen; Backbone Size:5365; Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:23 0
pFRT-TODestFLAGHA_RBPMS2
 
Resource Report
Resource Website 		RRID:Addgene_59390 RBPMS2 Homo sapiens Ampicillin PMID:24860013 Backbone Marker: Life Technologies, Modified by Tuschl Lab ; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 0
pcDNA3-Nrxn1beta AS4(-)-Fc
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59313 Nrxn1beta AS4(-)-Fc fusion Mus musculus Ampicillin PMID:10892652 Fc-portion contains intronic sequences which improve expression. See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion). The Nrxn 1 beta variant encoded by this plasmid is AS4(-), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent / skipped. Backbone Size:5381; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:22 5
P29-sp64 Poly A
 
Resource Report
Resource Website 		RRID:Addgene_59434 VZV ORF 29 human herpesvirus type 3 (varicella zoster virus) Ampicillin PMID:11090142 Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:23 0
pMXs-hGLIS1GR
 
Resource Report
Resource Website 		RRID:Addgene_59312 GLIS1 Homo sapiens Ampicillin PMID:24759836 Backbone Marker:Dr. Toshio Kitamura; Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:22 0
P21-sp64 Poly A
 
Resource Report
Resource Website 		RRID:Addgene_59433 VZV ORF 21 human herpesvirus type 3 (varicella zoster virus) Ampicillin PMID:11090142 Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:23 0
pcDNA3-Nrxn1beta AS4(+)-Fc
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_59314 Nrxn1beta AS4(+)-Fc fusion Mus musculus Ampicillin PMID:10892652 Fc-portion contains intronic sequences which improve expression. See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion). The Nrxn 1 beta variant encoded by this plasmid is AS4(+), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present. Backbone Size:6672; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:22 1
P63-sp64 Poly A
 
Resource Report
Resource Website 		RRID:Addgene_59435 VZV ORF 63 human herpesvirus type 3 (varicella zoster virus) Ampicillin PMID:11090142 Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:23 0
pUltra-Smurf-luc-RTTA3
 
Resource Report
Resource Website 		RRID:Addgene_59438 luciferase Ampicillin loxP5171 at positions after cppt and before WPRE Identical to pUltra-puro-luc-RTTA3 (Addgene plasmid 58764) but puro is replaced by AmCyan, for FACS Sorting instead of puro selection. Backbone Marker:Moore Lab, Addgene plasmid 48687; Vector Backbone:pUltra-Chili; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:23 0

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