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Species: Homo sapiens
Genetic Insert: MEG3c
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17569660
Proper citation: RRID:Addgene_59422 Copy
Species: Homo sapiens
Genetic Insert: hFOXH1 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #8452; Backbone Size:6700; Vector Backbone:pMKO.1-puro; Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24759836
Proper citation: RRID:Addgene_59303 Copy
Genetic Insert: IFP2.0T2AHO1 IRES EGFP
Vector Backbone Description: Backbone Marker:addgene; Backbone Size:9594; Vector Backbone:pLenti CMV/TO Puro DEST (670-1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24832154
Proper citation: RRID:Addgene_59427 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59382 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-dfrA
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Trimethoprim
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™
Proper citation: RRID:Addgene_59384 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-kan
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The kanamycin resistance gene was taken from pACYC177. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59383 Copy
Vector Backbone Description: Backbone Marker:Herring and Blattner; Backbone Size:7742; Vector Backbone:pKDTS; Vector Types:Bacterial Expression, Genome Engineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25255806
Comments: We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59386 Copy
Species: Synthetic
Genetic Insert: TT-ISceI-cat2
Vector Backbone Description: Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25255806
Comments: iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script.
Proper citation: RRID:Addgene_59385 Copy
Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker: Life Technologies, Modified by Tuschl Lab ; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24860013
Proper citation: RRID:Addgene_59388 Copy
Species: Homo sapiens
Genetic Insert: MEG3b
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17569660
Proper citation: RRID:Addgene_59421 Copy
Species: Homo sapiens
Genetic Insert: MEG3a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17569660
Proper citation: RRID:Addgene_59420 Copy
Species: Homo sapiens
Genetic Insert: RBPMS
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5365; Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24860013
Proper citation: RRID:Addgene_59391 Copy
Species: Homo sapiens
Genetic Insert: RBPMS2
Vector Backbone Description: Backbone Marker: Life Technologies, Modified by Tuschl Lab ; Backbone Size:5372; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24860013
Proper citation: RRID:Addgene_59390 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(-)-Fc fusion
Vector Backbone Description: Backbone Size:5381; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10892652
Comments: Fc-portion contains intronic sequences which improve expression.
See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion).
The Nrxn 1 beta variant encoded by this plasmid is AS4(-), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent / skipped.
Proper citation: RRID:Addgene_59313 Copy
Species: human herpesvirus type 3 (varicella zoster virus)
Genetic Insert: VZV ORF 29
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11090142
Proper citation: RRID:Addgene_59434 Copy
Species: Homo sapiens
Genetic Insert: GLIS1
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura; Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24759836
Proper citation: RRID:Addgene_59312 Copy
Species: human herpesvirus type 3 (varicella zoster virus)
Genetic Insert: VZV ORF 21
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11090142
Proper citation: RRID:Addgene_59433 Copy
Species: Mus musculus
Genetic Insert: Nrxn1beta AS4(+)-Fc fusion
Vector Backbone Description: Backbone Size:6672; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10892652
Comments: Fc-portion contains intronic sequences which improve expression.
See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.
Proper citation: RRID:Addgene_59314 Copy
Species: human herpesvirus type 3 (varicella zoster virus)
Genetic Insert: VZV ORF 63
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3030; Vector Backbone:pSP64 poly(A); Vector Types:makes synthetic transcripts that contains poly A; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11090142
Proper citation: RRID:Addgene_59435 Copy
Genetic Insert: luciferase
Vector Backbone Description: Backbone Marker:Moore Lab, Addgene plasmid 48687; Vector Backbone:pUltra-Chili; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: loxP5171 at positions after cppt and before WPRE
Identical to pUltra-puro-luc-RTTA3 (Addgene plasmid 58764) but puro is replaced by AmCyan, for FACS Sorting instead of puro selection.
Proper citation: RRID:Addgene_59438 Copy
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