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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pAT253 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40943 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253. | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | 2026-08-15 01:14:40 | 1 |
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pCMV5-Flag-EPLIN alpha delta domain 7 Resource Report Resource Website |
RRID:Addgene_40940 | EPLIN alpha delta Domain 7 | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 562-600 | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN alpha delta N112 Resource Report Resource Website |
RRID:Addgene_40935 | EPLIN | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 1-112 | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN alpha delta N54 Resource Report Resource Website |
RRID:Addgene_40934 | EPLIN | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 1-54 | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN alpha delta LIM Resource Report Resource Website |
RRID:Addgene_40933 | EPLIN alpha | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | LIM domain deleted- amino acids 221-295 | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN beta delta C term Resource Report Resource Website |
RRID:Addgene_40931 | EPLIN beta | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | C terminus deleted | 2026-08-15 01:14:40 | 0 | |
|
PM-FRB-mRFP-T2A-FKBP-5-ptase Resource Report Resource Website 1+ mentions |
RRID:Addgene_40896 | PM-FRB-mRFP-T2A-FKBP-5-ptase | Kanamycin | PMID:22357943 | Vector Backbone:pmRFP-C1; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:40 | 6 | |||
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pCMV5-Flag-EPLIN beta delta LIM domain Resource Report Resource Website |
RRID:Addgene_40938 | EPLIN beta delta LIM domain | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | LIM domain deleted | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN alpha delta domain 3 Resource Report Resource Website |
RRID:Addgene_40937 | EPLIN alpha delta Domain 3 | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 106-148 | 2026-08-15 01:14:40 | 0 | |
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pCMV5-Flag-EPLIN alpha delta N173 Resource Report Resource Website |
RRID:Addgene_40936 | EPLIN | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 1-173 | 2026-08-15 01:14:40 | 0 | |
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pLKO.1puro-shKIBRA-B Resource Report Resource Website 1+ mentions |
RRID:Addgene_40889 | shKIBRA-B | Homo sapiens | Ampicillin | PMID:22614006 | Backbone Marker:Addgene 8453; Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 1 | ||
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pBabepuro-KIBRA Resource Report Resource Website 1+ mentions |
RRID:Addgene_40887 | KIBRA | Homo sapiens | Ampicillin | PMID:22614006 | Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 2 | ||
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pLKO.1puro-shWillin-B Resource Report Resource Website |
RRID:Addgene_40886 | shWillin-B | Homo sapiens | Ampicillin | PMID:21666719 | Backbone Marker:Addgene 8453; Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 0 | ||
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pCMV5-Flag-EPLIN beta Resource Report Resource Website |
RRID:Addgene_40929 | EPLIN beta | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 0 | ||
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pCMV5-Flag-EPLIN alpha Resource Report Resource Website 1+ mentions |
RRID:Addgene_40928 | EPLIN alpha | Homo sapiens | Ampicillin | PMID:11950948 | Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 1 | ||
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pcDNA3-FLAG-ATBF1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40927 | ATBF1 | Homo sapiens | Ampicillin | PMID:20720010 | Addgene NGS results found S80A, V785A variants compared to the NCBI reference [NM_006885.4]. | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | See depositor comments below. | 2026-08-15 01:14:40 | 1 |
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pKXUa1-HA-ATBF1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40926 | ATBF1 | Homo sapiens | Ampicillin | PMID:20720010 | Backbone Marker:Kaiming Xu (Emory University); Backbone Size:6750; Vector Backbone:pKXUa1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 1 | ||
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pCS2+8NmOrange Resource Report Resource Website |
RRID:Addgene_40883 | Ampicillin | PMID:23124201 | Backbone Marker:Turner and Weintraub (1994); Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Sea urchin, zebrafish, Xenopus ; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:40 | 0 | ||||
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pcDNA3-Smac(1-60)mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_40880 | Smac(1-60)mCherry | Homo sapiens | Ampicillin | PMID:20493813 | Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:39 | 4 | ||
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pBad His6 mOCR TEV LIC cloning vector (8O) Resource Report Resource Website |
RRID:Addgene_37504 | Ampicillin | This plasmid is an empty vector. Your gene can be inserted via LIC cloning. 8-series vectors are induced with L-arabinose for tighter control of expression. Glucose can be added to the medium to further inhibit leaky expression. The plasmid can be expressed in any E. coli line that lacks proteases. 8O adds a TEV-cleavable His6-mOCR tag to the N-terminus of your protein. mOCR can enhance the solubility of your protein of interest. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ | Backbone Size:6053; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:15 | 0 |
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