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Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Addgene plasmid 25890; Vector Backbone:pLX304; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954536
Proper citation: RRID:Addgene_59144 Copy
Species: Mus musculus
Genetic Insert: Krt84-ex1
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59264 Copy
Species: Homo sapiens
Genetic Insert: PKA Kinase Translocation Reporter
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24949979
Proper citation: RRID:Addgene_59146 Copy
Species: Homo sapiens
Genetic Insert: p38 Kinase Translocation Reporter
Vector Backbone Description: Backbone Marker:Eric Campeau Lab (Addgene Plasmid 19068); Vector Backbone:pLENTI PGK Puro DEST (w529-2); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24949979
Proper citation: RRID:Addgene_59152 Copy
Species: Mus musculus
Genetic Insert: 1.5kb upstream krt5
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59281 Copy
Species: Mus musculus
Genetic Insert: Intergenic 2kb upstream krt18
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59280 Copy
Species: Mus musculus
Genetic Insert: Cbx1
Vector Backbone Description: Backbone Marker:van Steensel lab, Addgene plasmid 59204; Vector Backbone:pINDgw RFA-V5-EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17038565
Proper citation: RRID:Addgene_59203 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59205 Copy
Vector Backbone Description: Backbone Marker:van Steensal lab, Addgene plasmid 59201; Backbone Size:7660; Vector Backbone:pIND(V5)EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: Note that this vector can not be used to express Dam only.
Proper citation: RRID:Addgene_59204 Copy
Species: Mus musculus
Genetic Insert: Krt7-Intron 2
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59286 Copy
Species: Mus musculus
Genetic Insert: mFlg int-Intron 1
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59288 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Size:4368; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR.
Proper citation: RRID:Addgene_59171 Copy
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8780; Vector Backbone:pCasper-hs; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Comments: myc epitope tag serves as a linker peptide between dam and its fusion partner. This tag can be used to detect the fusion proteins by Western blotting or immunofluorescence microscopy to confirm their correct size and localization. Note that in order to obtain a detectable amount of protein, the fusion protein should be overexpressed by heat shock induction.
This vector cannot be used to express the myc-Dam protein by itself, since there is no startcodon.
Proper citation: RRID:Addgene_59218 Copy
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8780; Vector Backbone:pCasper-hs; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Comments: myc epitope tag serves as a linker peptide between dam and its fusion partner. This tag can be used to detect the fusion proteins by Western blotting or immunofluorescence microscopy to confirm their correct size and localization. Note that in order to obtain a detectable amount of protein, the fusion protein should be overexpressed by heat shock induction.
This vector can also be used to express the Dam-myc protein by itself (there is a stopcodon 15 aa after the myc-tag).
Proper citation: RRID:Addgene_59217 Copy
Species: Mus musculus
Genetic Insert: Lmo3.8 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21599714
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59294 Copy
Species: Synthetic
Genetic Insert: QuasAr2-Citrine eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59172 Copy
Species: Mus musculus
Genetic Insert: Lmo4.2 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20618444
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59293 Copy
Species: Synthetic
Genetic Insert: QuasAr2-mRuby2 eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59174 Copy
Species: Mus musculus
Genetic Insert: Lmo3.5 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21599714
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59295 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pPZP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25879861
Proper citation: RRID:Addgene_59177 Copy
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