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http://www.addgene.org/63821

Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589

Proper citation: RRID:Addgene_63821 Copy   

•   Source: Addgene

http://www.addgene.org/63822

Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24952589

Proper citation: RRID:Addgene_63822 Copy   

•   Source: Addgene

http://www.addgene.org/63828

Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546

Proper citation: RRID:Addgene_63828 Copy   

•   Source: Addgene

http://www.addgene.org/63784

Species: Synthetic
Genetic Insert: eSapphire-Strep-tag II followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: The sequence of the eSapphire gene has been optimized for S. cerevisiae and generated by gene synthesis. The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.

Proper citation: RRID:Addgene_63784 Copy   

•   Source: Addgene

http://www.addgene.org/63836

Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546

Proper citation: RRID:Addgene_63836 Copy   

•   Source: Addgene

http://www.addgene.org/63832

Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546

Proper citation: RRID:Addgene_63832 Copy   

•   Source: Addgene

http://www.addgene.org/63833

Species: Synthetic
Genetic Insert: CFP, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26117546

Proper citation: RRID:Addgene_63833 Copy   

•   Source: Addgene

http://www.addgene.org/63796

Species: Synthetic
Genetic Insert: CBP-3xFLAG-TEV-Protein A followed by KlLEU2
Vector Backbone Description: Backbone Marker:Fermentas/Thermo ; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25486077

Proper citation: RRID:Addgene_63796 Copy   

•   Source: Addgene

http://www.addgene.org/63791

Species: Synthetic
Genetic Insert: eGFP-Twin-Strep tag followed by URA3
Vector Backbone Description: Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Comments: Derived from plasmid pGS1212 by site-directed mutagenesis. The Twin-Strep tag sequence is adapted from the sequence of commercial Twin-Strep tag plasmids offered by IBA, Goettingen, Germany.

Proper citation: RRID:Addgene_63791 Copy   

•   Source: Addgene

http://www.addgene.org/63848

Species: Synthetic
Genetic Insert: No-aptamer control mRNA
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63848 Copy   

•   Source: Addgene

http://www.addgene.org/63856

Species: Synthetic
Genetic Insert: No-aptamer control mRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63856 Copy   

•   Source: Addgene

http://www.addgene.org/63857

Species: Synthetic
Genetic Insert: TMR7 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63857 Copy   

•   Source: Addgene

http://www.addgene.org/63858

Species: Synthetic
Genetic Insert: TMR10 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63858 Copy   

•   Source: Addgene

http://www.addgene.org/63852

Species: Synthetic
Genetic Insert: DNT3 DNT riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63852 Copy   

•   Source: Addgene

http://www.addgene.org/63850

Species: Synthetic
Genetic Insert: Theo45 theophylline riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63850 Copy   

•   Source: Addgene

http://www.addgene.org/63885

Species: Synthetic
Genetic Insert: ER-localized low-affinity GCaMP3(10.19)
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26451944

Proper citation: RRID:Addgene_63885 Copy   

•   Source: Addgene

http://www.addgene.org/63890

Species: Synthetic
Genetic Insert: SS Stuffer
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pComb3H; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10986398
Comments: pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control. Select References: Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin _v_3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody. FASEB, 16(14):2000-2. Berry, J.D.; Rutherford, J.; Silverman, G.J.; Kaul, R.; Elia, M.; Gobuty, S.; Fuller, R.; Plummer, F.A.; & Barbas III, C.F. (2003) Development of Functional Human Monoclonal Single-Chain Variable Fragment Antibody Against HIV-1 From Human Cervical B cells. Hybridoma and Hybridomics, 22(2):, 97-108. Jendreyko N, Popkov M, Beerli RR, Chung J, McGavern DB, Rader C, Barbas CF 3rd. (2003) Intra-diabodies: Bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. J Biol Chem., 278(48):, 47812-9. Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization. J. Biol. Chem., 275,, 36073-36078. Goncalves, J.; Kilva, F.; Freitas-Vieira, A.; Santa-Marta, M.; Malho, R.; Yang, X.; Gabuzda, D.; and Barbas III, C.F. (2002) Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem., 277(35):32036-45. Popkov, M.; Mage, R.G.; Alexander, C.B.; Thundivalappil, S.; Barbas III, C.F.; Rader, C. (2003) Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: The impact of Kappa Allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J. Mol. Biol., 325:325-335. Chung, J.; Rader, C.; Popkov, M.; Hur, Y.-M.; Kim, H.-K.; Lee, Y.-J.; & Barbas III, C. F. (2004) Integrin _IIb_3 specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation,FASEB 18(2):361-3. Popkov, M.; Jendreyko, N.; Gonzalez-Sapienza, G.; Mage, R.G.; Rader, C.; Barbas III, C.F. (2004) Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from animmune b9 allotype rabbit antibody library, J. Immunol. Methods, 288(1-2):149-164. Popkov, M.; Rader, C.; Barbas III, C.F. (2004) Isolation of human prostate cancer reactive antibodies using phage display technology, J. Immunol. Methods, 291:137-151. Tanaka, F.; Fuller, R.; Barbas III, C.F. (2005) Development of Small Designer Aldolase Enzymes: Catalytic Activity, Folding, and Substrate Specificity. Biochemistry, 44:7583-7592. This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (https://blog.addgene.org/plasmids-101-dimers-and-multimers)

Proper citation: RRID:Addgene_63890 Copy   

•   Source: Addgene

http://www.addgene.org/64074

Species: Synthetic
Genetic Insert: O-acetyl-L-homoserine sulfhydrylase
Vector Backbone Description: Backbone Marker:DNA 2.0; Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25884979
Comments: For expression of non-tagged oah2 only.

Proper citation: RRID:Addgene_64074 Copy   

•   Source: Addgene

http://www.addgene.org/64161

Species: Synthetic
Genetic Insert: sgRNA1 for Tet-inducible Luciferase reporter
Vector Backbone Description: Backbone Marker:Charles Gersbach, Addgene plasmid 47108; Vector Backbone:pSPgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936

Proper citation: RRID:Addgene_64161 Copy   

•   Source: Addgene

http://www.addgene.org/64201

Species: Synthetic
Genetic Insert: Hypoxanthine guanine xanthine phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22726686

Proper citation: RRID:Addgene_64201 Copy   

•   Source: Addgene