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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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mRFP1-21 Resource Report Resource Website |
RRID:Addgene_63821 | mRFP1 | Synthetic | Chloramphenicol | PMID:24952589 | Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Codon optimized for E.coli | 2026-08-15 01:18:06 | 0 | |
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mRFP1-29 Resource Report Resource Website |
RRID:Addgene_63822 | mRFP1 | Synthetic | Chloramphenicol | PMID:24952589 | Vector Backbone:FTV; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Codon optimized for E.coli | 2026-08-15 01:18:06 | 0 | |
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pTd-4-15-13-3 Resource Report Resource Website |
RRID:Addgene_63828 | CFP, mRFP1 and GFPmut3b | Synthetic | Chloramphenicol | PMID:26117546 | Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | The intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol . CFPRBS is around 1000 au | 2026-08-15 01:18:06 | 0 | |
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pGS1112 Resource Report Resource Website |
RRID:Addgene_63784 | eSapphire-Strep-tag II followed by URA3 | Synthetic | Ampicillin | PMID:24969434 | The sequence of the eSapphire gene has been optimized for S. cerevisiae and generated by gene synthesis. The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany. | Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:05 | 0 | |
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pTd-4-15-13-11 Resource Report Resource Website |
RRID:Addgene_63836 | CFP, mRFP1 and GFPmut3b | Synthetic | Chloramphenicol | PMID:26117546 | Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | The intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol. CFPRBS is around 200000 au | 2026-08-15 01:18:06 | 0 | |
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pTd-4-15-13-7 Resource Report Resource Website |
RRID:Addgene_63832 | CFP, mRFP1 and GFPmut3b | Synthetic | Chloramphenicol | PMID:26117546 | Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | The intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol. CFPRBS is around 30000 au | 2026-08-15 01:18:06 | 0 | |
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pTd-4-15-13-8 Resource Report Resource Website |
RRID:Addgene_63833 | CFP, mRFP1 and GFPmut3b | Synthetic | Chloramphenicol | PMID:26117546 | Vector Backbone:FTV3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | The intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol. CFPRBS is around 50000 au | 2026-08-15 01:18:06 | 0 | |
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pGS1034 Resource Report Resource Website |
RRID:Addgene_63796 | CBP-3xFLAG-TEV-Protein A followed by KlLEU2 | Synthetic | Ampicillin | PMID:25486077 | Backbone Marker:Fermentas/Thermo ; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:05 | 0 | ||
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pGS1222 Resource Report Resource Website |
RRID:Addgene_63791 | eGFP-Twin-Strep tag followed by URA3 | Synthetic | Ampicillin | PMID:24969434 | Derived from plasmid pGS1212 by site-directed mutagenesis. The Twin-Strep tag sequence is adapted from the sequence of commercial Twin-Strep tag plasmids offered by IBA, Goettingen, Germany. | Backbone Size:4940; Vector Backbone:pGS1212; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:05 | 0 | |
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AEB-No-aptamer-controlmRFP1 Resource Report Resource Website |
RRID:Addgene_63848 | No-aptamer control mRNA | Synthetic | Chloramphenicol | PMID:26621913 | Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:18:06 | 0 | ||
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Mishler2014-No-aptamer-controlluciferase-pUC19 Resource Report Resource Website |
RRID:Addgene_63856 | No-aptamer control mRNA | Synthetic | Ampicillin | PMID:26621913 | Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||
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AEB-TMR7pUC19 Resource Report Resource Website |
RRID:Addgene_63857 | TMR7 TMR riboswitch | Synthetic | Ampicillin | PMID:26621913 | Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||
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AEB-TMR10pUC19 Resource Report Resource Website |
RRID:Addgene_63858 | TMR10 TMR riboswitch | Synthetic | Ampicillin | PMID:26621913 | Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 0 | ||
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AEB-DNT3 Resource Report Resource Website |
RRID:Addgene_63852 | DNT3 DNT riboswitch | Synthetic | Chloramphenicol | PMID:26621913 | Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:18:06 | 0 | ||
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AEB-Theo45 Resource Report Resource Website 1+ mentions |
RRID:Addgene_63850 | Theo45 theophylline riboswitch | Synthetic | Chloramphenicol | PMID:26621913 | Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:18:06 | 2 | ||
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pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL Resource Report Resource Website 1+ mentions |
RRID:Addgene_63885 | ER-localized low-affinity GCaMP3(10.19) | Synthetic | Ampicillin | PMID:26451944 | Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:06 | 3 | ||
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pComb3XSS Resource Report Resource Website 10+ mentions |
RRID:Addgene_63890 | SS Stuffer | Synthetic | Ampicillin | PMID:10986398 | pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control. Select References: Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin _v_3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody. FASEB, 16(14):2000-2. Berry, J.D.; Rutherford, J.; Silverman, G.J.; Kaul, R.; Elia, M.; Gobuty, S.; Fuller, R.; Plummer, F.A.; & Barbas III, C.F. (2003) Development of Functional Human Monoclonal Single-Chain Variable Fragment Antibody Against HIV-1 From Human Cervical B cells. Hybridoma and Hybridomics, 22(2):, 97-108. Jendreyko N, Popkov M, Beerli RR, Chung J, McGavern DB, Rader C, Barbas CF 3rd. (2003) Intra-diabodies: Bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. J Biol Chem., 278(48):, 47812-9. Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization. J. Biol. Chem., 275,, 36073-36078. Goncalves, J.; Kilva, F.; Freitas-Vieira, A.; Santa-Marta, M.; Malho, R.; Yang, X.; Gabuzda, D.; and Barbas III, C.F. (2002) Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem., 277(35):32036-45. Popkov, M.; Mage, R.G.; Alexander, C.B.; Thundivalappil, S.; Barbas III, C.F.; Rader, C. (2003) Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: The impact of Kappa Allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J. Mol. Biol., 325:325-335. Chung, J.; Rader, C.; Popkov, M.; Hur, Y.-M.; Kim, H.-K.; Lee, Y.-J.; & Barbas III, C. F. (2004) Integrin _IIb_3 specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation,FASEB 18(2):361-3. Popkov, M.; Jendreyko, N.; Gonzalez-Sapienza, G.; Mage, R.G.; Rader, C.; Barbas III, C.F. (2004) Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from animmune b9 allotype rabbit antibody library, J. Immunol. Methods, 288(1-2):149-164. Popkov, M.; Rader, C.; Barbas III, C.F. (2004) Isolation of human prostate cancer reactive antibodies using phage display technology, J. Immunol. Methods, 291:137-151. Tanaka, F.; Fuller, R.; Barbas III, C.F. (2005) Development of Small Designer Aldolase Enzymes: Catalytic Activity, Folding, and Substrate Specificity. Biochemistry, 44:7583-7592. This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (https://blog.addgene.org/plasmids-101-dimers-and-multimers) | Backbone Size:3300; Vector Backbone:pComb3H; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:07 | 18 | |
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OAH2 Resource Report Resource Website |
RRID:Addgene_64074 | O-acetyl-L-homoserine sulfhydrylase | Synthetic | Kanamycin | PMID:25884979 | For expression of non-tagged oah2 only. | Backbone Marker:DNA 2.0; Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:08 | 0 | |
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sgRNA1_Tet-inducible Luciferase reporter Resource Report Resource Website 1+ mentions |
RRID:Addgene_64161 | sgRNA1 for Tet-inducible Luciferase reporter | Synthetic | Ampicillin | PMID:25619936 | Backbone Marker:Charles Gersbach, Addgene plasmid 47108; Vector Backbone:pSPgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:09 | 1 | ||
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HGXPRT Resource Report Resource Website 1+ mentions |
RRID:Addgene_64201 | Hypoxanthine guanine xanthine phosphoribosyltransferase | Synthetic | Ampicillin | PMID:22726686 | Backbone Marker:Invitrogen; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:09 | 1 |
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