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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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hGFAP-Cre Resource Report Resource Website 1+ mentions |
RRID:Addgene_40591 | Cre recombinase | Homo sapiens | Ampicillin | PMID:11668683 | This plasmid encodes a transgene to express the Cre recombinase in astrocytes of transgenic mice. A DNA fragment encoding the Cre recombinase was inserted into an expression cassette containing the gfa2 promoter (Besnard et al., 1991), a 2.2-kb 5' flanking region from the human GFAP (hGFAP) gene. The nuclear targeting signal from the SV40 large T antigen was inserted at the amino terminal end of the coding region, because it reportedly increases the efficiency of DNA excision by the recombinase (Gu et al., 1993). A heterologous intron and polyadenylation signal were provided by sequences from the mouse protamine 1 gene at the 3' end. The hGFAP-cre transgene was constructed by excising the lacZ coding region from pgfa2-lac2 (Brenner et al., 1994) by BamHI digestion, and replacing it by blunt end ligation with a nuclear-targeted Cre coding sequence. The latter was obtained as a 1.1-kb SalI-MluI fragment from plasmid pTZ19RCreNLS, which was kindly provided by Drs. Raul Torres and Klaus Rajewsky. Both junctions were verified by DNA sequencing. | Backbone Marker:Michael Brenner (PMID: 7952266); Backbone Size:5500; Vector Backbone:pgfa2-lac2; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin | Construct contains a nuclear-targeted Cre recombinase followed by a heterologous mouse intron and polyadenylation signal, all under the control of the gfa2 promoter | 2026-08-15 01:14:37 | 8 |
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PLXNA4A Resource Report Resource Website |
RRID:Addgene_40748 | PLXNA4A | Homo sapiens | Kanamycin | http://www.thesgc.org/structures/4E74/ | Backbone Marker:SGC; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:38 | 0 | ||
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FUGW-H1-Syndecan-1 shRNA (UTR) Resource Report Resource Website 1+ mentions |
RRID:Addgene_40623 | Syndecan-1 shRNA(UTR) | Mus musculus | Ampicillin | PMID:22936997 | This shRNA plasmid was generated by inserting the hairpin oligonucleotides into the FUGW-H1 lentiviral construct. | Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 2 | |
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pXL010-Wnt dual (GFP-Fire) reporter Resource Report Resource Website 1+ mentions |
RRID:Addgene_40588 | Wnt reporter sequences | Homo sapiens | Ampicillin | PMID:22177620 | This lentiviral Wnt reporter has 7 repeats of Tcf/Lef binding sites, which was cloned by Xiaojun (Lance) Lian. Methods for Plasmid construction, lentiviral production and infection of hESCs: The 7 TCF/LEF binding site sequences were obtained by direct PCR of plasmid M50 (Addgene plasmid 12456) and the GreenFire (GF) sequences were obtained by direct PCR of the pGreenFire1-mCMV Plasmid (System Bioscience TR010PA-1). These two sequences were cloned into pSicoR PGK puro (Addgene plasmid 11586) digested by Xba I (NEB) and Xho I (NEB). The constructed Wnt reporter plasmid was named 7TGFP and verified by sequencing. This 7TGFP vector was cotransfected with the helper plasmids psPAX2 and pMD2.G (Addgene plasmids 12260 and 12259) into HEK-293TN cells (System Biosciences) for virus production. Virus-containing medium was collected at 48 and 72 hours after transfection and used for infection of hESCs in the presence of 6μg/mL polybrene (Sigma). Transduced cells were cultured in mTeSR1 on Matrigel for three days and then clonally isolated in mTeSR1 with 1 μg/mL puromycin. | Backbone Marker:addgene; Vector Backbone:pSicoR PGK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 3 | |
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pcDNA3-HA-TCF1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40620 | TCF7 | Homo sapiens | Ampicillin | PMID:22949634 | Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 4 | ||
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PP-ASL Resource Report Resource Website |
RRID:Addgene_40749 | Trypanosoma Brucei Adenylosuccinate Lyase | Trypanosoma Brucei | Ampicillin | http://www.thesgc.org/structures/4EFC/ Tb427tmp.160.5560:MAC049-C01:C223709 | Backbone Marker:SGC; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 0 | ||
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pRMH124-VP16-CIB1 Resource Report Resource Website |
RRID:Addgene_40615 | VP16-CIB1 | Arabidopsis thaliana | Ampicillin | PMID:22922268 | Backbone Marker:Clontech; Backbone Size:7500; Vector Backbone:pGADT7rec; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 0 | ||
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719-2µ-minHIS3 Resource Report Resource Website |
RRID:Addgene_40610 | HIS3 | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6512; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Every codon of the coding sequence has been replaced with the isoaccepting codon decoded by the least abundant tRNA. | 2026-08-15 01:14:37 | 0 | |
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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b Resource Report Resource Website |
RRID:Addgene_40731 | Aurora B FRET-sensor | Synthetic | Ampicillin | PMID:22801782 | Backbone Marker:Clontech; Backbone Size:5125; Vector Backbone:pIRES-puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 0 | ||
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pMRBad-Z-CspGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_40730 | Leucine Zipper prime - C super positive Green Fluorescent Protein | Synthetic | Kanamycin | PMID:22692102 | Backbone Size:4496; Vector Backbone:pMRBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:38 | 5 | ||
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pCDH-F-PA1 Resource Report Resource Website |
RRID:Addgene_40618 | PA1 | Homo sapiens | Ampicillin | PMID:22949634 | Backbone Marker:System Biosciences (SBI); Backbone Size:8295; Vector Backbone:pCDH-EF1-MCS-IRES-Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 0 | ||
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RPRD1B Resource Report Resource Website |
RRID:Addgene_40739 | RPRD1B | Homo sapiens | Ampicillin | http://www.thesgc.org/structures/4FLA/ | Backbone Marker:SGC; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 0 | ||
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L3MBTL3 Resource Report Resource Website |
RRID:Addgene_40738 | L3MBTL3 | Homo sapiens | Kanamycin | http://www.thesgc.org/structures/4FL6/ | Backbone Marker:SGC; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:38 | 0 | ||
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AMPK-γ1-HA Resource Report Resource Website 1+ mentions |
RRID:Addgene_40605 | AMPK-γ1 | Mus musculus | Kanamycin | PMID:17012231 | AMPK-γ1 insert has Kozak sequence embedded at 5'-terminus and a stop codon at the 3'-terminus. The FLAG of the pCMV-Tag4a backbone is out of frame. | Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:37 | 1 | |
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AMPK-β2-FLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_40603 | AMPK-β2 | Mus musculus | Kanamycin | PMID:17012231 | Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:37 | 1 | ||
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AMPK-β1-FLAG Resource Report Resource Website |
RRID:Addgene_40602 | AMPK-β1 | Mus musculus | Kanamycin | PMID:17012231 | Backbone Size:4300; Vector Backbone:pCMV-Tag4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:37 | 0 | ||
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pTH731-2µ-RLuc/minCFLuc Resource Report Resource Website 1+ mentions |
RRID:Addgene_40601 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6518; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | All codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:37 | 1 | |
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pEMB018 LRRK2_BC_1520_2148_K1906M Resource Report Resource Website |
RRID:Addgene_40567 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB108; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | aa1520_2148; K1906M | 2026-08-15 01:14:37 | 0 | ||
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pTH646-2µ-RLuc Resource Report Resource Website 1+ mentions |
RRID:Addgene_40600 | Renilla Luciferase | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6518; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 1 | ||
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pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEK Resource Report Resource Website |
RRID:Addgene_40565 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB52; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1520_2210 | 2026-08-15 01:14:37 | 0 |
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