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Species: Homo sapiens
Genetic Insert: PDB:4NW2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4NW2/
http://www.thesgc.org/structures/4O42/
Proper citation: RRID:Addgene_58755 Copy
Species: Homo sapiens
Genetic Insert: PDB:4O62
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4O62/
Proper citation: RRID:Addgene_58758 Copy
Species: Homo sapiens
Genetic Insert: PDB:4PWY
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4PWY/
Proper citation: RRID:Addgene_58761 Copy
Species: Aquorea victoria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3976; Vector Backbone:pJ401; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Alternative name: p006-kan-GFP-T5lac Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_58534 Copy
Genetic Insert: dtA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24828191
Proper citation: RRID:Addgene_58536 Copy
Species: Aquorea victoria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:3979; Vector Backbone:pJ401; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Alternative name: p007-amp-GFP-T5lac Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_58535 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Comments: Note: Addgene's quality control sequencing has found an R318G amino acid residue substitution in CD4. The affect on protein function is not known.
Proper citation: RRID:Addgene_58538 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24627473
Proper citation: RRID:Addgene_58537 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Proper citation: RRID:Addgene_58542 Copy
Vector Backbone Description: Backbone Size:7270; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58545 Copy
Vector Backbone Description: Backbone Size:7489; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58547 Copy
Species: Mus musculus
Genetic Insert: ptc
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS+; Vector Types:Bacterial Expression, in situ probe; Bacterial Resistance:Ampicillin
Comments: For sense: linearize with HindIII, transcribe with T7.
For anti-sense: linearize with BamHI, transcribe with T3.
Proper citation: RRID:Addgene_58701 Copy
Vector Backbone Description: Backbone Size:7859; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58549 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17641202
Proper citation: RRID:Addgene_58703 Copy
Vector Backbone Description: Backbone Size:7473; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58548 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Proper citation: RRID:Addgene_58540 Copy
Species: Mus musculus
Genetic Insert: Ptch
Vector Backbone Description: Vector Backbone:pMal; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17641202
Comments: See antibody production protocol in supplemental documents.
Proper citation: RRID:Addgene_58705 Copy
Species: Homo sapiens
Genetic Insert: shPIK3CD
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 8453) ; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24449900
Comments: The primers were generated by the TRC (#TRCN0000033276) Construct mildly knocks down p110d (PIK3CD) protein expression ~2 fold after 3 X 500 uL infection in MCF10A-5E cells.
The specificity of this construct has not been confirmed by addback, but a second hairpin against the same target yielded the same phenotype.
Proper citation: RRID:Addgene_58707 Copy
Species: Clostridium thermocellum
Genetic Insert: CBM-CohI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25194847
Proper citation: RRID:Addgene_58709 Copy
Species: Synthetic
Genetic Insert: sfGFP-DocI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25194847
Proper citation: RRID:Addgene_58708 Copy
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