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Species: Homo sapiens
Genetic Insert: HTT partial exon 1 Q23
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10528852
Proper citation: RRID:Addgene_40261 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40381 Copy
Species: Synthetic
Genetic Insert: Clover GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5410; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Clover is the brightest green fluorescent protein (GFP) characterized to date, with robust performance in protein fusions. Also, it is useful as a FRET donor to mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap.
Proper citation: RRID:Addgene_40259 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40379 Copy
Species: Synthetic
Genetic Insert: VSFP-CR
Vector Backbone Description: Backbone Marker:Invitrogen/Dr. Paulmurugan Ramasamy; Backbone Size:6107; Vector Backbone:pcDNA3.1/Puro-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Voltage sensor domain of Ci-VSP fused to a pair of fluorescent proteins consisting of a Clover GFP and mRuby2 RFP.
Proper citation: RRID:Addgene_40257 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40378 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40377 Copy
Species: Synthetic
Genetic Insert: AKAR2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5489; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: ECFP was replaced with GFP Clover and Citrine was replaced with RFP mRuby2.
Note: there is a small sequence insertion downstream of the insert that adds restriction sites. This is not present in the full plasmids sequence, so please refer to Addgene's QC sequence with the BGH-Rev primer for additional information.
Proper citation: RRID:Addgene_40255 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40376 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_40374 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Gm cassette (BssHII+SphI)
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:18353436
Comments: NS1 vector
Gm resistant version of pAM1303.
Both pAM1303 and pAM3511 were digested with BssHII and SphI, and then mix-ligated. The accC1gene (Gm) replaces the aadA gene (SpSm) in the Ω cassette. The upstream T4 terminator was lost due to SphI digestion.
Proper citation: RRID:Addgene_40252 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus4x/cdc37; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Co-expresses cdc37
Proper citation: RRID:Addgene_40373 Copy
Species: Anabaena sp. Strain PCC 7120
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pAM505; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9794762
Comments: Contain promoterless gfp from pKEN2-GFPmut2 inserted into pAM505.
HindIII-Klenow fill-SacI fragment of gfp is inserted into SmaI-SacI digested pAM505. This is a control of gfp without a promoter. The multiple cloning site upstream the gfp gene includes: SalI, SacI, KpnI,Asp718, XmaI, and SmaI.
Proper citation: RRID:Addgene_40251 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus4x/cdc37; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Co-expresses cdc37
Proper citation: RRID:Addgene_40370 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus4x/cdc37; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Co-expresses cdc37
Proper citation: RRID:Addgene_40368 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Polylinker
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16079339
Comments: NS1 vector
cloning sites: NotI, SacII, BglII, SnaBI, BsiWI, MluI, EcoRI, XhoI, StuI, SpeI, and BamHI.
additional unique sites inserted between the NotI and BamHI sites of the NSI vector pAM1303:
5’-NotI-SacII-BglII-SnaBI-BsiWI-MluI-EcoRI-XhoI-StuI-SpeI-BamHI-3’
Proper citation: RRID:Addgene_40246 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus4x/cdc37; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Co-expresses cdc37
Proper citation: RRID:Addgene_40367 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI::luxCDE, PpsbAI::luxAB
Vector Backbone Description: Vector Backbone:pAM1582; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17417005
Comments: NSII.1 vector
Lux Genes: luxAB & luxCDE
synthesizes luciferase and substrate in S. elongatus
SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.
Proper citation: RRID:Addgene_40245 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pBACgus4x/cdc37; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin
Comments: Co-expresses cdc37
Proper citation: RRID:Addgene_40366 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI
Vector Backbone Description: Vector Backbone:pAM1667; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NS2.1 vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942
500bp PvuII-SalI promoter fragment of psbAI inserted into SalI-StuI digested pAM1667 (luxCDE).
Proper citation: RRID:Addgene_40242 Copy
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