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Species: C. reinhardtii
Genetic Insert: ChR2(C128S)-eGFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 22051; Backbone Size:9200; Vector Backbone:FUGW (from Addgene plasmid 22051); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: Please see Addgene plasmid 20294 (pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE) for the original version of this gene (under the CamKII promoter).
Proper citation: RRID:Addgene_58510 Copy
Species: Chloromonas oogama
Genetic Insert: CoChR(C108S)-eGFP
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: CoChR was published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_58512 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: Please see Addgene plasmid 40755 for more information on GCaMP6f; also note that GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58514 Copy
Species: Scherffelia dubia
Genetic Insert: sdChR(C138S)-TS-eGFP-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: sdChR was published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_58513 Copy
Species: Homo sapiens
Genetic Insert: Utr261
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26317264
Proper citation: RRID:Addgene_58471 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26317264
Proper citation: RRID:Addgene_58470 Copy
Species: Chloromonas oogama
Genetic Insert: CoChR(C108S)-GCaMP6f-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: CoChR was published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58521 Copy
Species: Halorubrum sodomense
Genetic Insert: Archaerhodopsin-3 (with C-terminal His tag)
Vector Backbone Description: Backbone Size:5269; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23530193
Proper citation: RRID:Addgene_58487 Copy
Species: Scherffelia dubia
Genetic Insert: sdChR(C138S)-TS-GCaMP6f-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: sdChR was first published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58522 Copy
Species: Other
Genetic Insert: neo
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002522
Proper citation: RRID:Addgene_58525 Copy
Species: Scherffelia dubia
Genetic Insert: sdChR(C138S, E154A)-TS-GCaMP6f-ER
Vector Backbone Description: Backbone Size:9240; Vector Backbone:FCK(1.3)GW, from Addgene 22217; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296307
Comments: sdChR was first published in: Klapoetke, N. C., Y. Murata, S.S. Kim, S.R. Pulver, A. Birdsey-Benson, Y.K. Cho, T.K. Morimoto, A.S. Chuong, E.J. Carpenter and Z. Tian. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
sdChR(E154A) was first published in: Hochbaum, Zhao, Farhi, Klapoetke, et al. 2014. "All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins." Nature Methods (in press).
GCaMP6f was published in: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.)
Proper citation: RRID:Addgene_58524 Copy
Species: Other
Genetic Insert: neo
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002522
Proper citation: RRID:Addgene_58526 Copy
Species: Mus musculus
Genetic Insert: JunD
Vector Backbone Description: Backbone Marker:Weinberg Lab (Addgene plasmid 1767) ; Backbone Size:5300; Vector Backbone:pBabe neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: pBabe JunD_HA neo was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe neo, which had been digested with BamHI and EcoRI and then dephosphorylated.
Proper citation: RRID:Addgene_58486 Copy
Species: T. gondii
Genetic Insert: DHFR cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4555; Vector Backbone:pDEST R4-R3 Vector II; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24825012
Proper citation: RRID:Addgene_58528 Copy
Species: Mus musculus
Genetic Insert: IFITM3
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22511783
Proper citation: RRID:Addgene_58413 Copy
Species: Corynactis sp.
Genetic Insert: Red fluorescent protein
Vector Backbone Description: Backbone Marker:DNA20; Backbone Size:3929; Vector Backbone:pJ401; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Alternative name: p005-kan-RFP-T5lac Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_58533 Copy
Species: Mus musculus
Genetic Insert: IFITM1
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23804635
Proper citation: RRID:Addgene_58415 Copy
Species: Homo sapiens
Genetic Insert: KRT5
Vector Backbone Description: Backbone Size:6265; Vector Backbone:pBabe N-terminal RFP hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24658685
Comments: pBabe RFP1_KRT5 hygro was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe N-terminal RFP hygro. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe N-terminal RFP hygro, which had been digested with BamHI and EcoRI and then dephosphorylated.
Proper citation: RRID:Addgene_58493 Copy
Species: Homo sapiens
Genetic Insert: IRE1a
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23487760
Proper citation: RRID:Addgene_58492 Copy
Genetic Insert: encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Vector Backbone Description: Vector Backbone:pRL542; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3148842
Comments: MobColK, M.AvaI, M.Eco47II
Please see attached map for more information on how this vector was constructed and cloning details.
Proper citation: RRID:Addgene_58495 Copy
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