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http://www.addgene.org/40146

Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (C56S)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR C56S

Proper citation: RRID:Addgene_40146 Copy   

•   Source: Addgene

http://www.addgene.org/40141

Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + 7SA)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + 7SA 7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A Note: A deletion of amino acid residues 502-523 near the C-terminus of the protein was discovered during the Addgene quality control process and is present in this plasmid.

Proper citation: RRID:Addgene_40141 Copy   

•   Source: Addgene

http://www.addgene.org/40137

Genetic Insert: par-2 RNAi resistant (wild-type)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR wild-type

Proper citation: RRID:Addgene_40137 Copy   

•   Source: Addgene

http://www.addgene.org/40132

Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4814; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747757
Comments: For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar Please note that plasmid #40132 is only functional in combination with plasmid #40131.

Proper citation: RRID:Addgene_40132 Copy   

•   Source: Addgene

http://www.addgene.org/40083

Species: E. coli
Genetic Insert: gusA
Vector Backbone Description: Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22820336
Comments: GenBank accession number JQ895026 Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA

Proper citation: RRID:Addgene_40083 Copy   

•   Source: Addgene

http://www.addgene.org/40124

Species: Homo sapiens
Genetic Insert: RINT1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7732; Vector Backbone:pLenti6-V5/DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23074196

Proper citation: RRID:Addgene_40124 Copy   

•   Source: Addgene

http://www.addgene.org/40121

Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa244-468)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa245-STOP (RNAi res) +3’UTR

Proper citation: RRID:Addgene_40121 Copy   

•   Source: Addgene

http://www.addgene.org/40120

Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa1-244) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa1-245 (RNAi res) +3’UTR

Proper citation: RRID:Addgene_40120 Copy   

•   Source: Addgene

http://www.addgene.org/40086

Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa 345 - 468)
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004). Alternate plasmid name: pIC26 Dendra + MEX-5 (aa345-STOP) (Dendra + MEX-5 ORF)

Proper citation: RRID:Addgene_40086 Copy   

•   Source: Addgene

http://www.addgene.org/40084

Species: jellyfish
Genetic Insert: gfpmut 3.1
Vector Backbone Description: Backbone Size:4768; Vector Backbone:pBBRMCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:22820336
Comments: GenBank accession number: JQ895027 pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame. This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites

Proper citation: RRID:Addgene_40084 Copy   

•   Source: Addgene

http://www.addgene.org/40238

Species: Synechococcus elongatus PCC 7942
Genetic Insert: psbAI promoter, luxCDE
Vector Backbone Description: Vector Backbone:pAM1504; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10812624
Comments: NS1 vector Lux Genes: luxCDE Directs luciferase substrate synthesis in PCC7942. psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria.

Proper citation: RRID:Addgene_40238 Copy   

•   Source: Addgene

http://www.addgene.org/40237

Species: Synechococcus elongatus PCC 7942
Genetic Insert: promoterless luxAB reporter
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10812624
Comments: NS1 vector Lux Genes: luxAB cloning sites NotI and BamHI Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites.

Proper citation: RRID:Addgene_40237 Copy   

•   Source: Addgene

http://www.addgene.org/40236

Species: Synthetic
Genetic Insert: LOV-ipaA
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5442; Vector Backbone:pET-21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22520757
Comments: This vector encodes a photo-activable caged form of the vinculin binding ipaA peptide. Regarding the hybridized region, the first ten residues of the ipaA VBS1 helical peptide were identified as a close match to the last ten residues of the AsLOV2 Jα helix; five of the ten positions are identical, and the hydrophobic residues on the Jα helix that make critical contacts with the AsLOV2 domain beta-sheet at residues 539, 542, and 543 are conserved in the alignment with ipaA. Additionally, residues in the ipaA sequence that make extensive contacts with vinculin are conserved in the alignment (Ile 612, Ala 615, Ala 616, and Val 619 in ipaA). Side-chain optimization simulations were used to thread the first ten residues of ipaA onto the last ten residues of the Jα helix. The 540 position on the Jα helix was converted to isoleucine. The LOV-ipaA gene was synthesized with a six histidine N-terminal tag (Genscript, Piscataway, NJ, USA) and cloned into the pET21b vector. Protein coding sequence of LOV-ipaA: MHHHHHHGSLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTANNIIKAAKDVTTSLSKVLKNIN

Proper citation: RRID:Addgene_40236 Copy   

•   Source: Addgene

http://www.addgene.org/40235

Genetic Insert: yEGFP1
Vector Backbone Description: Backbone Size:5667; Vector Backbone:pRS413; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: yEGFP contains a M233I point mutation. Depositor states that this mutation does not affect yEGFP function.

Proper citation: RRID:Addgene_40235 Copy   

•   Source: Addgene

http://www.addgene.org/40355

Species: Mus musculus
Genetic Insert: CD40L
Vector Backbone Description: Backbone Marker:Addgene plasmid 40569; Vector Backbone:MIEG-hCD4; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19405121
Comments: The CD40-ligand (CD40L)-expressing retroviral vector was constructed by PCR amplification of cDNA from anti-CD3 activated mouse T cells with the following primers for CD40-ligand: sense 5'-CTTTCAGTCAGCATGATAGAAACA-3' and antisense 5'-TCAGAGTTTGAGTAAGCCAAAAGA-3'; these primers were used to amplify the complete coding region of mouse CD40 ligand. The CD40-ligand gene was then reamplified with primers containing XhoI and NotI sites and then cloned via XhoI and NotI sites into a retroviral vector expressing human CD4.

Proper citation: RRID:Addgene_40355 Copy   

•   Source: Addgene

http://www.addgene.org/40350

Species: Homo sapiens
Genetic Insert: Jun dominant negative
Vector Backbone Description: Backbone Marker:David A. Williams (PMID: 10961859); Vector Backbone:pMIEG3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15699140
Comments: The Jun dominant negative vector was made by first preparing a version of c-Jun with the first 122 aa deleted. This cDNA was amplified by PCR from human c-Jun with the following primers: sense, 5-AAAAAA-GAATTC-ATGACTAGCCAGAACACGCTGCCCAGCGTC-3; antisense, 5-AAAAAA-GAATTC-TCAGCTGGCATAGTCAGGCACGTCATAAGGATAGCTAAATGTTTGCAAC-3. The antisense primer adds a hemagglutinin tag to the C terminus of c-Jun. The PCR product was then cut with EcoRI and cloned into the retroviral vector pMIEG3.

Proper citation: RRID:Addgene_40350 Copy   

•   Source: Addgene

http://www.addgene.org/40346

Species: Homo sapiens
Genetic Insert: BCL6
Vector Backbone Description: Backbone Marker:Niwa, Yamamura, and Miyazaki (PMID: 1660837); Backbone Size:5200; Vector Backbone:pCXN2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12165517
Comments: CXN-BCL-6 was constructed by inserting the human BCL-6 cDNA from pBS-BCL-6 full length (FL) via SalI ends into the XhoI site in the pCXN2 expression vector. The pCXN2 (CXN) vector was obtained from Dr. K. Ozato (National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD) and consists of the human BCL-6 cDNA driven by a hybrid
β-actin-CMV promoter.

Proper citation: RRID:Addgene_40346 Copy   

•   Source: Addgene

http://www.addgene.org/40344

Species: Homo sapiens
Genetic Insert: RASSF6
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Comments: pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI. A digest with NheI and SalI will excise the insert with tags.

Proper citation: RRID:Addgene_40344 Copy   

•   Source: Addgene

http://www.addgene.org/40343

Species: Rous sarcoma virus (RSV)
Genetic Insert: RSV promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12165517
Comments: The Rous sarcoma virus (RSV) promoter reporter was constructed by isolating the RSV promoter from pRc/RSV (Invitrogen) via BglII and HindIII sites and cloned into pGL3-basic by the same sites.

Proper citation: RRID:Addgene_40343 Copy   

•   Source: Addgene

http://www.addgene.org/40342

Genetic Insert: 3xAP-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12165517
Comments: The 3 AP-1 reporter contains three canonical AP-1 binding sites (TGACTCA) upstream of a minimal promoter fragment containing a TATA box in the luciferase reporter plasmid pGL3-basic.

Proper citation: RRID:Addgene_40342 Copy   

•   Source: Addgene