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Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
Proper citation: RRID:Addgene_21595 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21511 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21509 Copy
Species: Caenorhabditis elegans
Genetic Insert: BiP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)
Proper citation: RRID:Addgene_21896 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::mCherry (w introns w/o stop)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2646; Vector Backbone:pDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_22513 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26387 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP::unc-54_3'UTR
Vector Backbone Description: Backbone Size:10813; Vector Backbone:pBCN27-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729840
Comments: The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.
Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.
Proper citation: RRID:Addgene_26347 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7 hairpin only
Vector Backbone Description: Backbone Size:6946; Vector Backbone:MDH1-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26488 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7 (~250nt flanking each side)
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7060; Vector Backbone:MXW-PGK-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: Ordinarily, we express miRNAs from the LTR, which is pol III. However, cel-let-7 won't express from that promoter (possibly due to long poly-T tracts). This is a pol II expression system.
Proper citation: RRID:Addgene_26491 Copy
Species: Caenorhabditis elegans
Genetic Insert: pptr-1
Vector Backbone Description: Backbone Marker:Jason Pellettieri ; Backbone Size:8356; Vector Backbone:pMAL-c2X (MBP fusion); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_131374 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-1
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6p1 (GST-fusion); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_131371 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-1
Vector Backbone Description: Backbone Marker:Jason Pellettieri ; Backbone Size:8356; Vector Backbone:pMAL-c2X (MBP fusion); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_131372 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-4
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6p1 (GST-fusion); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_131370 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3 (aa545-862)
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27914198
Proper citation: RRID:Addgene_131368 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3 (aa1-544)
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27914198
Proper citation: RRID:Addgene_131367 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16a
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7430; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_132367 Copy
Species: Caenorhabditis elegans
Genetic Insert: INS-6
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8291; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Comments: Plasmid contains the cDNA of ins-6 based on wormbase sequence.
Proper citation: RRID:Addgene_132365 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNG-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Proper citation: RRID:Addgene_132523 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122253 Copy
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