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  • Organism:arabidopsis thaliana (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
AtCOPT2 upGFP
 
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RRID:Addgene_117901 AtCOPT2 upGFP Arabidopsis thaliana Bleocin (Zeocin) insert amino acid sequence: MDHDHMHDMPPPSPSSSSMSNHTTPHMMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIVIFLLAVIAEWLAHSPILRVSGSTNRAAGLAQTAVYTLKTGLSYLVMLAVMSFNAGVFIVAIAGYGVGFFLFGSTTFKKPSDDQKTAELLPPSSGCVCENLYQSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSSHHHHHH Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:59 0
AtCOPT2 uPIC
 
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RRID:Addgene_117900 AtCOPT2 uPIC Arabidopsis thaliana Bleocin (Zeocin) insert amino acid sequence: MDHDHMHDMPPPSPSSSSMSNHTTPHMMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIVIFLLAVIAEWLAHSPILRVSGSTNRAAGLAQTAVYTLKTGLSYLVMLAVMSFNAGVFIVAIAGYGVGFFLFGSTTFKKPSDDQKTAELLPPSSGCVCENLYQSHHHHHH Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:58 0
AtCOPT1 upGFP
 
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RRID:Addgene_117899 AtCOPT1 upGFP Arabidopsis thaliana Bleocin (Zeocin) insert amino acid sequence: MDHDHMHGMPRPSSSSSSSPSSMMNNGSMNEGGGHHHMKMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSANRAAGLIQTAVYTLRIGLAYLVMLAVMSFNAGVFLVALAGHAVGFMLFGSQTFRNTSDDRKTNYVPPSGCACENLYQSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSSHHHHHH Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:58 0
AtCOPT1 uPIC
 
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RRID:Addgene_117898 AtCOPT1 uPIC Arabidopsis thaliana Bleocin (Zeocin) insert amino acid sequence: MDHDHMHGMPRPSSSSSSSPSSMMNNGSMNEGGGHHHMKMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSANRAAGLIQTAVYTLRIGLAYLVMLAVMSFNAGVFLVALAGHAVGFMLFGSQTFRNTSDDRKTNYVPPSGCACENLYQSHHHHHH Backbone Size:3329; Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:58 0
pESC/CURT_fluoB
 
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RRID:Addgene_117997 CURT_fluoB Arabidopsis thaliana Ampicillin PMID:30884945 Backbone Marker:Agilent Technologies; Backbone Size:6571; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, Yeast/bacteria binary vector; Bacterial Resistance:Ampicillin 2026-08-15 01:02:59 0
pBAD/CURT_fluoB
 
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RRID:Addgene_117999 CURT_fluoB Arabidopsis thaliana Ampicillin PMID:30884945 Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:59 0
pN_35S/CTP-mCitrine
 
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1+ mentions
RRID:Addgene_117989 CTP-mCitrine Arabidopsis thaliana Spectinomycin PMID:30884945 mCitrine contains an L7E compared to the published mCitrine sequence that does not impact plasmid function. Backbone Size:9204; Vector Backbone:pN_35S; Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:59 1
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
 
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RRID:Addgene_110145 Csy4 Arabidopsis thaliana Spectinomycin PMID:28094975 Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:01:37 0
pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
 
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RRID:Addgene_110143 Csy4 Arabidopsis thaliana Spectinomycin PMID:28094975 Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression, binary vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:01:37 0
INCTbiosyn-p35S-GFP(rc)4
 
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1+ mentions
RRID:Addgene_127522 EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 4 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S-GFP(rc)2
 
Resource Report
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1+ mentions
RRID:Addgene_127521 EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 2 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S(rc)2_4_5-GFP
 
Resource Report
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1+ mentions
RRID:Addgene_127520 CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The CaMV 35S promoter in a reverse complement sequence orientation came from iGEM registry (BBa_K1547006 ) and the attB/P integrases 2, 4 and 5 attatchment sites flanking this sequence came from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). This cassete was previously synthesized in pBluescript II SK(-) and after cloned using HindIII and BamHI replacing the forward CaMV 35S promoter sequence from a 35S-GFP plasmid construction of Chiu, W. et al. Curr. Biol. 6, 325-330 (1996). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:36 1
INCTbiosyn-p35S-GFP(rc)7
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_127524 EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 7 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S-GFP(rc)Bxb1
 
Resource Report
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1+ mentions
RRID:Addgene_127528 EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase Bxb1 attachment sites sequences from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529327.1); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pBluescript SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
pFH6_new
 
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RRID:Addgene_105866 U6-26p::sgRNA scaffold Arabidopsis thaliana Ampicillin PMID:34541122 The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%. For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( http://www.bio-protocol.org/e2384 ). Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pGEM T-easy; Vector Types:Plant Expression, CRISPR, Synthetic Biology, Subcloning; Bacterial Resistance:Ampicillin 2026-08-15 01:00:56 0
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
 
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RRID:Addgene_106895 GAI-tagBFP-Sp dCas9-ABI Arabidopsis thaliana Ampicillin PMID:27776111 Backbone Marker:System Biosciences; Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac; Bacterial Resistance:Ampicillin 2026-08-15 01:01:04 0
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)
 
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RRID:Addgene_126026 CIBN-MP; CRY2 PHRolig-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin CRY2 E490G 2026-08-15 01:04:21 0
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
 
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RRID:Addgene_126024 CIBN-MP; CRY2 PHR-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin WT 2026-08-15 01:04:21 0
ARF17 pBluescript
 
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RRID:Addgene_12068 ARF17 Arabidopsis thaliana Ampicillin PMID:15829600 The genomic sequence of ARF17 (At1g77850), including 1.9 and 1.7 kb of putative 5' and 3' regulatory sequences, respectively, was cloned as an 6.6-kb EcoRI-SpeI fragment into pBluescript II SK+ (Stratagene, La Jolla, CA) from the BAC F28K19. See "sequence" for full insert sequence. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:25 0
5mCUC1 pGreenII0129
 
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RRID:Addgene_12067 CUC1 mutant Arabidopsis thaliana Kanamycin PMID:15202996 See "sequence" for full insert sequence. See article for cloning details. Backbone Size:5000; Vector Backbone:pGreenII0129; Vector Types:plant transformation via Agrobacterium; Bacterial Resistance:Kanamycin miR-resistant CUC1. Made by introducing five silent mutations within the miR164-complementarity domain of a CUC1 genomic clone. 2026-08-15 01:03:27 0

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