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Species: Synthetic
Genetic Insert: NG-HD dimer RVD at Position8
Vector Backbone Description: Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34480028
Comments: RVD is inserted into TA site of TetR T vector
Proper citation: RRID:Addgene_173399 Copy
Species: Synthetic
Genetic Insert: NG-HD dimer RVD at Position6
Vector Backbone Description: Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34480028
Comments: RVD is inserted into TA site of TetR T vector
Proper citation: RRID:Addgene_173397 Copy
Species: Synthetic
Genetic Insert: NG-HD dimer RVD at Position5
Vector Backbone Description: Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34480028
Comments: RVD is inserted into TA site of TetR T vector
Proper citation: RRID:Addgene_173396 Copy
Species: Synthetic
Genetic Insert: NG-HD dimer RVD at Position3
Vector Backbone Description: Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34480028
Comments: RVD is inserted into TA site of TetR T vector
Proper citation: RRID:Addgene_173394 Copy
Species: Synthetic
Genetic Insert: NG-HD dimer RVD at Position2
Vector Backbone Description: Vector Backbone:TetR T vector; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34480028
Comments: RVD is inserted into TA site of TetR T vector
Proper citation: RRID:Addgene_173393 Copy
Vector Backbone Description: Backbone Marker:Spaink; Backbone Size:10400; Vector Backbone:pMP103; Vector Types:Bacterial Expression, IncP; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24276795
Comments: the polylinker of pIC20H is broadly available and isn't covered by an MTA
Proper citation: RRID:Addgene_185921 Copy
Species: Synthetic
Genetic Insert: lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
Vector Backbone Description: Vector Backbone:pFW11 Tet; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37406096
Proper citation: RRID:Addgene_199119 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39919271
Proper citation: RRID:Addgene_231317 Copy
Species: n/a
Genetic Insert: pyrF-/hisB-/tnaA-
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39610115
Comments: Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent.
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
Proper citation: RRID:Addgene_228512 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker.
Proper citation: RRID:Addgene_212119 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker.
Proper citation: RRID:Addgene_212122 Copy
Genetic Insert: SUMO-TAG35-sfGFP
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) SUMO-sfGFP with an amber (TAG) codon at E35 within the SUMO coding region and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). This plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from the previous Ma version by containing a tetracycline efflux marker. The SUMO linker is included in order to distance the TAG site from sfGFP.
Proper citation: RRID:Addgene_212120 Copy
Vector Backbone Description: Backbone Size:4297; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers tetracycline resistance
Proper citation: RRID:Addgene_225161 Copy
Vector Backbone Description: Backbone Size:4533; Vector Backbone:pHBJT019; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39244484
Comments: pHBJT019 containing IPTG-inducible mCherry
Proper citation: RRID:Addgene_225178 Copy
Vector Backbone Description: Backbone Size:4532; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC18 MCS, confers tetracycline resistance
Proper citation: RRID:Addgene_225171 Copy
Vector Backbone Description: Backbone Size:4533; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS, confers tetracycline resistance
Proper citation: RRID:Addgene_225170 Copy
Genetic Insert: sfYFP
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248095 Copy
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248096 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Nova Lifetech Inc.; Backbone Size:10682; Vector Backbone:pRK415; Vector Types:Bacterial Expression, Synthetic Biology, very low copy; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248204 Copy
Species: Synthetic
Genetic Insert: PylRS-C6a
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pGS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41974833
Comments: Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint.
Proper citation: RRID:Addgene_251555 Copy
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