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pOS1T-chPylRS4OxzA
 
Resource Report
Resource Website 		RRID:Addgene_253663 chPylRS-4OxzA Synthetic Tetracycline PMID:41885601 Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint. Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:36:09 0
pOS1T-Mb(IPYE)PylRSτMH
 
Resource Report
Resource Website 		RRID:Addgene_253665 Mb(IPYE)PylRS-τMH Tetracycline PMID:41885601 Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint. Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:36:07 0
pOS1T-MmPylRS(8_2)
 
Resource Report
Resource Website 		RRID:Addgene_253670 MmPylRS(8_2) Synthetic Tetracycline PMID:41885601 Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint. Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:36:09 0
pOS1T-chPylR2E5MH
 
Resource Report
Resource Website 		RRID:Addgene_253657 chPylRS-2E5MH Synthetic Tetracycline PMID:41885601 Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint. Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:36:09 0
US0 pyrF-/hisB-/tnaA-
 
Resource Report
Resource Website 		RRID:Addgene_228512 pyrF-/hisB-/tnaA- n/a Tetracycline PMID:39610115 Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent. Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Tetracycline 2026-08-15 01:31:50 0
pNDGG004
 
Resource Report
Resource Website 		RRID:Addgene_231317 Tetracycline PMID:39919271 Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:32:04 0
pCnRed
 
Resource Report
Resource Website 		RRID:Addgene_232908 gam Synthetic Tetracycline PMID:39989320 Vector Backbone:pSa; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:33:07 0
pNDMS237
 
Resource Report
Resource Website 		RRID:Addgene_248095 sfYFP Tetracycline Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:34:43 0
pNDMS239
 
Resource Report
Resource Website 		RRID:Addgene_248096 mScarlet-I Tetracycline Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:34:44 0
pJS1
 
Resource Report
Resource Website 		RRID:Addgene_253726 dcas9 Streptococcus pasteurianus Tetracycline PMID:41670347 Vector Backbone:miniCTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:35:31 0
pMVV207
 
Resource Report
Resource Website 		RRID:Addgene_215343 S. stutzeri nifL gRNA Synthetic Tetracycline PMID:37988619 Vector Backbone:RSF1010; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:29:51 0
pSW003-PpsbA-mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_205019 mNeonGreen Synthetic Tetracycline PMID:29449848 Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline mNeonGreen is codon optimized for expression in P. fluorescens 2026-08-15 01:27:45 0
pSW002-PpsbA-mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_205017 mNeonGreen Synthetic Tetracycline PMID:29449848 Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline mNeonGreen is codon optimized for expression in P. fluorescens 2026-08-15 01:27:48 0
pMflT-o4-mNG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_201855 mNeonGreen Synthetic Tetracycline The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected. Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:27:52 1
pJLG039
 
Resource Report
Resource Website 		RRID:Addgene_192982 T7 polymerase E. coli Tetracycline PMID:36645284 The T7 polymerase gene (1) was cloned from the chromosome of BL21(DE3). Backbone Size:8751; Vector Backbone:pSW213; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:26:34 0
pMflT-o4-YFP
 
Resource Report
Resource Website 		RRID:Addgene_201856 LanYFP Synthetic Tetracycline Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:26:45 0
pALS3-Ma-sfGFP-WT
 
Resource Report
Resource Website 		RRID:Addgene_212119 SUMO-sfGFP-WT Tetracycline This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:30:28 0
pALS3-Ma-SUMO-sfGFP-WT
 
Resource Report
Resource Website 		RRID:Addgene_212122 SUMO-sfGFP-WT Tetracycline This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:30:28 0
pALS3-Ma-SUMO-TAG35-sfGFP
 
Resource Report
Resource Website 		RRID:Addgene_212120 SUMO-TAG35-sfGFP Tetracycline This plasmid encodes expression of a) SUMO-sfGFP with an amber (TAG) codon at E35 within the SUMO coding region and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). This plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from the previous Ma version by containing a tetracycline efflux marker. The SUMO linker is included in order to distance the TAG site from sfGFP. Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline E35TAG (within SUMO encoding sequence) 2026-08-15 01:30:28 0
pHBJT010
 
Resource Report
Resource Website 		RRID:Addgene_225161 Tetracycline PMID:39244484 Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers tetracycline resistance Backbone Size:4297; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:30:32 0

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