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Species: Synthetic
Genetic Insert: chPylRS-4OxzA
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253663 Copy
Genetic Insert: Mb(IPYE)PylRS-τMH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253665 Copy
Species: Synthetic
Genetic Insert: MmPylRS(8_2)
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253670 Copy
Species: Synthetic
Genetic Insert: chPylRS-2E5MH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253657 Copy
Species: n/a
Genetic Insert: pyrF-/hisB-/tnaA-
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39610115
Comments: Derived from US0 pyrF-/hisB- (Scot Wolfe, Addgene #12614). This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor. This strain is also has a deletion in tnaA gene and does not produce indole, verified by Kovac's Reagent.
Please visit https://doi.org/10.1101/2024.08.07.607023 for bioRxiv preprint.
Proper citation: RRID:Addgene_228512 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39919271
Proper citation: RRID:Addgene_231317 Copy
Species: Synthetic
Genetic Insert: gam
Vector Backbone Description: Vector Backbone:pSa; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39989320
Proper citation: RRID:Addgene_232908 Copy
Genetic Insert: sfYFP
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248095 Copy
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Barney Geddes; Vector Backbone:pNDGG004 (Addgene #231317); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_248096 Copy
Species: Streptococcus pasteurianus
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:miniCTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41670347
Proper citation: RRID:Addgene_253726 Copy
Species: Synthetic
Genetic Insert: S. stutzeri nifL gRNA
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37988619
Proper citation: RRID:Addgene_215343 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205019 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
Proper citation: RRID:Addgene_201855 Copy
Species: E. coli
Genetic Insert: T7 polymerase
Vector Backbone Description: Backbone Size:8751; Vector Backbone:pSW213; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36645284
Comments: The T7 polymerase gene (1) was cloned from the chromosome of BL21(DE3).
Proper citation: RRID:Addgene_192982 Copy
Species: Synthetic
Genetic Insert: LanYFP
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_201856 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sfGFP-WT, uninterrupted by TAG codons, and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This control plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker.
Proper citation: RRID:Addgene_212119 Copy
Genetic Insert: SUMO-sfGFP-WT
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker.
Proper citation: RRID:Addgene_212122 Copy
Genetic Insert: SUMO-TAG35-sfGFP
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) SUMO-sfGFP with an amber (TAG) codon at E35 within the SUMO coding region and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). This plasmid is used during fluorescence-based selection and characterization of one or more MaRS mutants. It is distinguished from the previous Ma version by containing a tetracycline efflux marker. The SUMO linker is included in order to distance the TAG site from sfGFP.
Proper citation: RRID:Addgene_212120 Copy
Vector Backbone Description: Backbone Size:4297; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers tetracycline resistance
Proper citation: RRID:Addgene_225161 Copy
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