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Species: Homo sapiens
Genetic Insert: cullin 7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547496
Proper citation: RRID:Addgene_20695 Copy
Species: Artificial
Genetic Insert: GFP-NLS-lacZ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10337476
Proper citation: RRID:Addgene_20701 Copy
Species: artificial gene
Genetic Insert: 4 finger ZFP
Vector Backbone Description: Backbone Size:3710; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_20704 Copy
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19243080
Proper citation: RRID:Addgene_20780 Copy
Species: Mus musculus
Genetic Insert: beta-transducin repeat containing (BTRC)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11245432
Comments: *possible Y377I mutation in stock. This mutation is not known to affect function.
Proper citation: RRID:Addgene_20718 Copy
Species: Homo sapiens
Genetic Insert: RING finger protein 1 (ROC1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17079684
Proper citation: RRID:Addgene_20717 Copy
Species: Homo sapiens
Genetic Insert: VHL
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7585187
Proper citation: RRID:Addgene_20790 Copy
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19243080
Proper citation: RRID:Addgene_20762 Copy
Species: Homo sapiens
Genetic Insert: HOXB5
Vector Backbone Description: Backbone Size:4820; Vector Backbone:pCMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_20983 Copy
Genetic Insert: rtTA-ON-Adv
Vector Backbone Description: Backbone Size:6262; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20910 Copy
Genetic Insert: miR30(P) targets
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12554881
Proper citation: RRID:Addgene_20875 Copy
Species: Homo sapiens
Genetic Insert: MLL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:pMSCV-neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060922
Comments: This is a control construct testing the newly developed fMLLpl construct for transforming activity in a MLL-ENL fusion.
SmaI/XhoI fragment from pBluescriptII-ENL3' was inserted into a PmlI/XhoI cut pMSCV-flagMLLpl vector. The end plasmid has ~4.18kb MLL + pl remainings + ~0.4kb ENL with remaining pBSII.
Proper citation: RRID:Addgene_20873 Copy
Species: E.coli
Genetic Insert: KanamycinResistance-RpsL
Vector Backbone Description: Backbone Size:2935; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:19160076
Proper citation: RRID:Addgene_20871 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of DNA binding 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19896442
Proper citation: RRID:Addgene_20964 Copy
Genetic Insert: Firefly luciferase shRNA
Vector Backbone Description: Backbone Size:7471; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_20962 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20960 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18474618
Comments: Addgene has detected several mutations in the UTR region of this construct.
Proper citation: RRID:Addgene_20977 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19896442
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.
Proper citation: RRID:Addgene_20971 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19896442
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Strawberry cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20970 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9250; Vector Backbone:pLenti-CaMKIIa; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17410168
Comments: Please see http://www.optogenetics.org
for additional information.
H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20944 Copy
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