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Species: Synthetic
Genetic Insert: monomeric streptavidin
Vector Backbone Description: Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Comments: Please note the YPet fluorescent protein present in the vector backbone is not expressed with the streptavidin insert.
Proper citation: RRID:Addgene_39860 Copy
Species: Mus musculus
Genetic Insert: Neurotrophin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39981 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin, EGFP, TM helix
Vector Backbone Description: Backbone Marker:Life Technology; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39863 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin fused to EGFP
Vector Backbone Description: Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39862 Copy
Species: Rattus norvegicus
Genetic Insert: TrkB.T1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI.
See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant.
Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32
Proper citation: RRID:Addgene_39980 Copy
Species: Mus musculus
Genetic Insert: Slp2-a C2AB
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40058 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40056 Copy
Species: Mus musculus
Genetic Insert: Slp2-a E11A/R32A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40055 Copy
Species: Mus musculus
Genetic Insert: Slp2-a C2B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40053 Copy
Species: Mus musculus
Genetic Insert: Slp2-a C2A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40052 Copy
Species: Homo sapiens
Genetic Insert: hSlp2-a C2AB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40051 Copy
Species: Homo sapiens
Genetic Insert: C11orf51
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5-FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21926987
Comments: Can be used to rescue siC11orf51-4 from Pines lab.
C11orf51 and mRuby are separated by IRES2 and therefore are not fused.
Proper citation: RRID:Addgene_39858 Copy
Species: Rattus norvegicus
Genetic Insert: TrkC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide
Please note that the endogenous signal sequence of TrkC was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequence for GenBank ID NM_001270656.1, the TrkC protein sequence starts at amino acid #32
Proper citation: RRID:Addgene_39979 Copy
Species: Homo sapiens
Genetic Insert: C11orf51
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5-FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21926987
Proper citation: RRID:Addgene_39859 Copy
Species: Homo sapiens
Genetic Insert: Emi1
Vector Backbone Description: Vector Backbone:pT7-Venus-C1; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17485488
Proper citation: RRID:Addgene_39854 Copy
Species: Homo sapiens
Genetic Insert: Cyclin A2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11285279
Proper citation: RRID:Addgene_39856 Copy
Species: Rattus norvegicus
Genetic Insert: TrkA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9927421
Comments: cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide
Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30
Proper citation: RRID:Addgene_39977 Copy
Species: Homo sapiens
Genetic Insert: CyclinB1
Vector Backbone Description: Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20404109
Proper citation: RRID:Addgene_39852 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40046 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a I18A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40043 Copy
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