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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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LaminA-C-18 Resource Report Resource Website |
RRID:Addgene_58193 | LaminA | Homo sapiens | Kanamycin | With SNAP tag. | Vector Backbone:pSNAPf-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:14 | 0 | ||
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PDHA1-N-10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_58195 | PDHA1 | Homo sapiens | Kanamycin | With SNAP tag. | Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:14 | 1 | ||
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pZE21-APX(W41F) dimeric Resource Report Resource Website |
RRID:Addgene_58199 | pea APX W41F | Synthetic | Kanamycin | PMID:24803433 | This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags. This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation. The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630). | Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | W41F | 2026-08-15 01:17:14 | 0 |
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pGLTR-X-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_58245 | attR1-Cm(R)-CcdB-attR2 | GATEWAY DEST cassette | Chloramphenicol and Ampicillin | PMID:24841113 | There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid. | Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:17:14 | 1 | |
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pGLTR-FP-GFP Resource Report Resource Website |
RRID:Addgene_58242 | attR1-Cm(R)-CcdB-attR2 | GATEWAY DEST cassette | Chloramphenicol and Ampicillin | PMID:24841113 | There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid. | Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:17:14 | 0 | |
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pET14b-PITPalpha K61A Resource Report Resource Website 1+ mentions |
RRID:Addgene_58258 | PITP alpha | Homo sapiens | Ampicillin | PMID:14962392 | Backbone Marker:EMD Millipore; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K61A (abolishes PI binding) | 2026-08-15 01:17:14 | 1 | |
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pAdSh.U6.gRNAS1 Resource Report Resource Website |
RRID:Addgene_58252 | U6.gRNAS1 cassette | Synthetic | Kanamycin | PMID:24870050 | Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:14 | 0 | ||
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pAdSh.U6.gRNAGFP Resource Report Resource Website |
RRID:Addgene_58255 | U6.gRNAGFP cassette | Synthetic | Kanamycin | PMID:24870050 | Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:14 | 0 | ||
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HSC1-GiP Resource Report Resource Website 1+ mentions |
RRID:Addgene_58254 | Enhanced Green Fluorescence Protein, Puromycin resistance gene | Ampicillin | PMID:23752310 | Please note that there is a small deletion (32bp) just after the 3’LTR inside the cloning vector that removes the M13R primer region. The depositing lab was able to produce viruses with good titer and doesn’t think the missing 32bp is a major concern. | Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:14 | 1 | ||
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tdTomato-ZO1-C-14 Resource Report Resource Website |
RRID:Addgene_58147 | ZO1 | Homo sapiens | Kanamycin | . Excitation = 554; Emission = 581 | Backbone Size:4750; Vector Backbone:tdTomato-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:13 | 0 | ||
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pCAG-HA-rat Nrxn2beta AS4(-) Resource Report Resource Website |
RRID:Addgene_58268 | Nrxn2beta AS4(-) | Rattus norvegicus | Ampicillin | PMID:17360903 | See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I). Insert can also be released by single digest using XhoI. The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4). Note: HA-epitope tag was introduced at the mature N-terminus. | Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:14 | 0 | |
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pCAG-HA-rat Nrxn1beta AS4(-) Resource Report Resource Website 1+ mentions |
RRID:Addgene_58267 | Nrxn1beta AS4(-) | Rattus norvegicus | Ampicillin | PMID:17360903 | See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI). Insert can also be released by single digest using XhoI. The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]). Note: HA-epitope tag was introduced at the mature N-terminus. | Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | see comments | 2026-08-15 01:17:14 | 2 |
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EGFP-Actin-3 Resource Report Resource Website |
RRID:Addgene_58149 | Actin | Homo sapiens | Kanamycin | . Excitation = 554; Emission = 581 | Backbone Size:4750; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:13 | 0 | ||
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tdTomato-Zyxin-6 Resource Report Resource Website |
RRID:Addgene_58148 | Zyxin | Homo sapiens | Kanamycin | PMID:18057027 | . Excitation = 554; Emission = 581 | Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:13 | 0 | |
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pBabe-Neuroserpin oloop Resource Report Resource Website |
RRID:Addgene_58260 | Neuroserpin | Homo sapiens | Ampicillin | PMID:24581498 | Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Mutant of neuroserpin lacking five residues from Gly231 to Ala235 | 2026-08-15 01:17:14 | 0 | |
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pLVX-FADD-DD Resource Report Resource Website 1+ mentions |
RRID:Addgene_58263 | FADD | Homo sapiens | Ampicillin | PMID:24581498 | Backbone Marker:Clontech; Backbone Size:8519; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | version of FADD (FADD-DD) that contains the FADD death domain (aa30-124), but lacks the death effector domain that binds to caspase-8 | 2026-08-15 01:17:14 | 4 | |
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pCAG-HA-rat Nrxn1alpha Resource Report Resource Website 1+ mentions |
RRID:Addgene_58266 | Nrxn1alpha | Rattus norvegicus | Ampicillin | PMID:17360903 | See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI). Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest). The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]). Note: HA-epitope tag was introduced at the mature N-terminus. | Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | see comments | 2026-08-15 01:17:14 | 3 |
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Turquoise-Venus-FRET-10 Resource Report Resource Website |
RRID:Addgene_58158 | Kanamycin | FRET Indicator - Live and Fixed Cells. | Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:13 | 0 | ||||
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pRSETC PITPbeta N89F Resource Report Resource Website |
RRID:Addgene_58279 | PITP beta | Homo sapiens | Ampicillin | PMID:20332109 | Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid. | Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N89F (abolishes PI binding) | 2026-08-15 01:17:14 | 0 |
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PS-CFP2-mCherry-FRET-10 Resource Report Resource Website |
RRID:Addgene_58157 | Kanamycin | FRET Indicator - Live and Fixed Cells. Excitation = 490 / 587; Emission = 511 / 610 | Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:13 | 0 |
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