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Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_39819 Copy
Species: Canis lupus familiaris (dog)
Genetic Insert: Slp4-a shRNA-2
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40070 Copy
Species: Homo sapiens
Genetic Insert: CD8β M-1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18490743
Proper citation: RRID:Addgene_40078 Copy
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pIC26 + wormanized Dendra:speI
Gene of interest should be cloned into the SpeI restriction site to generate Dendra2 FPAP2 N-terminal fusion proteins.
Proper citation: RRID:Addgene_40076 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S458A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S458A)
Proper citation: RRID:Addgene_40075 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_39997 Copy
Species: Homo sapiens
Genetic Insert: FZR1
Vector Backbone Description: Backbone Marker:Amersham; Vector Backbone:pGEX-6T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note that this construct has an additional mutation in Cdh1 - R52G.
Proper citation: RRID:Addgene_39877 Copy
Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pKC30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6088551
Comments: pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_39993 Copy
Species: Homo sapiens
Genetic Insert: CyclinB1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11907280
Proper citation: RRID:Addgene_39871 Copy
Species: Homo sapiens
Genetic Insert: CyclinB1(R42A)
Vector Backbone Description: Vector Backbone:pVenus-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070128
Proper citation: RRID:Addgene_39873 Copy
Species: Synthetic
Genetic Insert: Erk activity reporter
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23882122
Comments: A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
Proper citation: RRID:Addgene_39994 Copy
Species: Canis lupus familiaris (dog)
Genetic Insert: Slp4-a shRNA-1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40069 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a V21A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40067 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a I18A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40066 Copy
Species: Homo sapiens
Genetic Insert: hSlp4-a W118S
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40065 Copy
Species: Mus musculus
Genetic Insert: Slp2-a E11A/R32A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4900; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22820376
Proper citation: RRID:Addgene_40063 Copy
Genetic Insert: iRFP protein GAF domain (mutated)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39868 Copy
Species: Synthetic
Genetic Insert: monomeric streptavidin
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pYD1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22806584
Proper citation: RRID:Addgene_39865 Copy
Genetic Insert: iRFP protein PAS domain
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39867 Copy
Genetic Insert: iRFP protein PAS domain
Vector Backbone Description: Backbone Marker:Wegerer, A. et al., BMC Biotechnol 8, 2 (2008); Vector Backbone:pWA21 (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23891149
Proper citation: RRID:Addgene_39866 Copy
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