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http://www.addgene.org/58193

Species: Homo sapiens
Genetic Insert: LaminA
Vector Backbone Description: Vector Backbone:pSNAPf-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.

Proper citation: RRID:Addgene_58193 Copy   

•   Source: Addgene

http://www.addgene.org/58195

Species: Homo sapiens
Genetic Insert: PDHA1
Vector Backbone Description: Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.

Proper citation: RRID:Addgene_58195 Copy   

•   Source: Addgene

http://www.addgene.org/58199

Species: Synthetic
Genetic Insert: pea APX W41F
Vector Backbone Description: Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24803433
Comments: This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags. This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation. The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630).

Proper citation: RRID:Addgene_58199 Copy   

•   Source: Addgene

http://www.addgene.org/58245

Species: GATEWAY DEST cassette
Genetic Insert: attR1-Cm(R)-CcdB-attR2
Vector Backbone Description: Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.

Proper citation: RRID:Addgene_58245 Copy   

•   Source: Addgene

http://www.addgene.org/58242

Species: GATEWAY DEST cassette
Genetic Insert: attR1-Cm(R)-CcdB-attR2
Vector Backbone Description: Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.

Proper citation: RRID:Addgene_58242 Copy   

•   Source: Addgene

http://www.addgene.org/58258

Species: Homo sapiens
Genetic Insert: PITP alpha
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14962392

Proper citation: RRID:Addgene_58258 Copy   

•   Source: Addgene

http://www.addgene.org/58252

Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:

Proper citation: RRID:Addgene_58252 Copy   

•   Source: Addgene

http://www.addgene.org/58255

Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:

Proper citation: RRID:Addgene_58255 Copy   

•   Source: Addgene

http://www.addgene.org/58254

Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Comments: Please note that there is a small deletion (32bp) just after the 3’LTR inside the cloning vector that removes the M13R primer region. The depositing lab was able to produce viruses with good titer and doesn’t think the missing 32bp is a major concern.

Proper citation: RRID:Addgene_58254 Copy   

•   Source: Addgene

http://www.addgene.org/58147

Species: Homo sapiens
Genetic Insert: ZO1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581

Proper citation: RRID:Addgene_58147 Copy   

•   Source: Addgene

http://www.addgene.org/58268

Species: Rattus norvegicus
Genetic Insert: Nrxn2beta AS4(-)
Vector Backbone Description: Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I). Insert can also be released by single digest using XhoI. The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4). Note: HA-epitope tag was introduced at the mature N-terminus.

Proper citation: RRID:Addgene_58268 Copy   

•   Source: Addgene

http://www.addgene.org/58267

Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI). Insert can also be released by single digest using XhoI. The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]). Note: HA-epitope tag was introduced at the mature N-terminus.

Proper citation: RRID:Addgene_58267 Copy   

•   Source: Addgene

http://www.addgene.org/58149

Species: Homo sapiens
Genetic Insert: Actin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581

Proper citation: RRID:Addgene_58149 Copy   

•   Source: Addgene

http://www.addgene.org/58148

Species: Homo sapiens
Genetic Insert: Zyxin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18057027
Comments: . Excitation = 554; Emission = 581

Proper citation: RRID:Addgene_58148 Copy   

•   Source: Addgene

http://www.addgene.org/58260

Species: Homo sapiens
Genetic Insert: Neuroserpin
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498

Proper citation: RRID:Addgene_58260 Copy   

•   Source: Addgene

http://www.addgene.org/58263

Species: Homo sapiens
Genetic Insert: FADD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8519; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498

Proper citation: RRID:Addgene_58263 Copy   

•   Source: Addgene

http://www.addgene.org/58266

Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI). Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest). The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]). Note: HA-epitope tag was introduced at the mature N-terminus.

Proper citation: RRID:Addgene_58266 Copy   

•   Source: Addgene

http://www.addgene.org/58158

Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.

Proper citation: RRID:Addgene_58158 Copy   

•   Source: Addgene

http://www.addgene.org/58279

Species: Homo sapiens
Genetic Insert: PITP beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332109
Comments: Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid.

Proper citation: RRID:Addgene_58279 Copy   

•   Source: Addgene

http://www.addgene.org/58157

Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells. Excitation = 490 / 587; Emission = 511 / 610

Proper citation: RRID:Addgene_58157 Copy   

•   Source: Addgene