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Genetic Insert: T7-RNA polymerase
Vector Backbone Description: Backbone Size:3262; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Proper citation: RRID:Addgene_170101 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985758
Proper citation: RRID:Addgene_170100 Copy
Genetic Insert: sgBFP
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170183 Copy
Species: Stigmatella aurantica
Genetic Insert: Sa163 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170184 Copy
Species: Homo sapiens
Genetic Insert: ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170136 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cas5
Vector Backbone Description: Backbone Size:5221; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170090 Copy
Species: E. coli
Genetic Insert: beta-galactosidase with stuffer
Vector Backbone Description: Backbone Size:30000; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16407 Copy
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pAdTrack-CMV is used for expression of transgenes under a CMV promoter when a GFP tracer is desired.
Max insert size = 5.0kb with pAdEasy1 and 7.7kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16405 Copy
Species: Homo sapiens
Genetic Insert: Split GFP and YFP PM- Mt-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164110 Copy
Vector Backbone Description: Backbone Size:8320; Vector Backbone:pAdTrack; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pAdTrack is used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
Max insert size = 5.9kb with pAdEasy1 and 8.6kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16404 Copy
Species: Homo sapiens
Genetic Insert: Split GFP Po-Mt Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164115 Copy
Vector Backbone Description: Backbone Size:7452; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
See AdEasy Protocol linked in the supplemental documents section above for more information.
NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.
Proper citation: RRID:Addgene_16403 Copy
Species: Homo sapiens
Genetic Insert: Split GFP Po-Mt Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164116 Copy
Vector Backbone Description: Backbone Size:6736; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16402 Copy
Species: Homo sapiens
Genetic Insert: Split YFP Po-ER Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164113 Copy
Vector Backbone Description: Backbone Size:30767; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9482916
Comments: Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-2.
Plasmids recombined in pAdEasy-2 should be packaged in 911E4 cells to make virus.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16401 Copy
Species: Homo sapiens
Genetic Insert: Split YFP Po-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164114 Copy
Species: Homo sapiens
Genetic Insert: Split GFP and YFP Mt-ER- Po Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164117 Copy
Genetic Insert: PGK-NeoR & ZeoR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene.
The sequence information is the sequence between linkers A and B.
See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Neo: neomycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
Proper citation: RRID:Addgene_16413 Copy
Species: Homo sapiens
Genetic Insert: PTEN Neo targeting
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4000; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: This targeting vector yields 1-5% homologous recombinants in HCT116 and other cell lines. See author's map for more information.
Proper citation: RRID:Addgene_16411 Copy
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