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Species: Homo sapiens
Genetic Insert: TFAM Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human TFAM in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_159745 Copy
Genetic Insert: donor plasmid targeting sgRNA
Vector Backbone Description: Backbone Marker:Ralf Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Plasmid #64324); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33203764
Proper citation: RRID:Addgene_159742 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159863 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159864 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159862 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159860 Copy
Species: Caenorhabditis elegans
Genetic Insert: intron GG1 - 250bp
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159877 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159872 Copy
Species: Caenorhabditis elegans
Genetic Insert: [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159870 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Modified from the pHEE401E T-DNA vector described by Wang &al. (2015) Genome Biol 16: 144, doi:10.1186/s13059-015-0715-0: the Hygromycin selection maker of the T-DNA was exchanged with a cassette enabling selection on the basis of YFP-fluorescence in seeds. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253203v1 for BioRxiv preprint
Proper citation: RRID:Addgene_159750 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159809 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159808 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159801 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33093202
Comments: Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
Proper citation: RRID:Addgene_159762 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15976 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CYC1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4173; Vector Backbone:pRS404; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15973 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159817 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159814 Copy
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