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Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pDSB_0011; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0056 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109087 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0057 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109088 Copy
Species: Synthetic
Genetic Insert: mTFP1
Vector Backbone Description: Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0032 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109080 Copy
Species: Homo sapiens
Genetic Insert: ANGPTL3
Vector Backbone Description: Backbone Size:4989; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Comments: 21bp deletion in the SV40 promoter sequence. May affect blasticidin selection.
Proper citation: RRID:Addgene_109112 Copy
Species: Homo sapiens
Genetic Insert: ANGPTL8
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Proper citation: RRID:Addgene_109114 Copy
Species: Homo sapiens
Genetic Insert: ANGPTL8
Vector Backbone Description: Backbone Size:8031; Vector Backbone:pRRL-cPPT-MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Proper citation: RRID:Addgene_109116 Copy
Species: Synthetic
Genetic Insert: mito-gTEMP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28212432
Proper citation: RRID:Addgene_109117 Copy
Species: Synthetic
Genetic Insert: nuc-gTEMP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28212432
Proper citation: RRID:Addgene_109118 Copy
Species: Homo sapiens
Genetic Insert: CTNNB1 Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTNNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQASNSAVDGTAAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_109119 Copy
Species: Synthetic
Genetic Insert: PTSG_00306 geranyl-geranylation signal
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0104 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109096 Copy
Species: Synthetic
Genetic Insert: mTFP1_3'UTR Act
Vector Backbone Description: Vector Backbone:pDSB_0050; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0093 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109097 Copy
Genetic Insert: eiC++-mRuby2-ST
Vector Backbone Description: Backbone Size:5833; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109132 Copy
Genetic Insert: ePsuACR-mRuby2-ST
Vector Backbone Description: Backbone Size:5837; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109133 Copy
Species: Synthetic
Genetic Insert: mCherry_SV40 NLS
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0059 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109091 Copy
Species: B. subtilis
Genetic Insert: HA1
Vector Backbone Description: Backbone Marker:Michelle Calos; Vector Backbone:pDY-, pBR322 derivative; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29294101
Comments: Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_109123 Copy
Genetic Insert: C1V1-TT-mRuby2-ST
Vector Backbone Description: Backbone Size:5832; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109124 Copy
Genetic Insert: ChR2-mRuby2-ST
Vector Backbone Description: Backbone Size:5852; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109125 Copy
Species: Mus musculus
Genetic Insert: ChroME(F243Y)-mRuby2-ST
Vector Backbone Description: Backbone Size:5855; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109127 Copy
Genetic Insert: ChroME(S273A)-mRuby2-ST
Vector Backbone Description: Backbone Size:5855; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29713079
Proper citation: RRID:Addgene_109128 Copy
Vector Backbone Description: Backbone Size:2287; Vector Backbone:pUC57; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_109220 Copy
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