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Species: Homo sapiens
Genetic Insert: Myocilin wild-type
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19959812
Proper citation: RRID:Addgene_39326 Copy
Species: synthetic gene
Genetic Insert: MSP1E3D1_D73C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19903557
Proper citation: RRID:Addgene_39328 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.
Proper citation: RRID:Addgene_39324 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39285 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39284 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: Use GST reverse primer to verify mutation in nmt1 promoter
Proper citation: RRID:Addgene_39286 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39292 Copy
Species: Neisseria meningitidis
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:pET-based custom vector; Backbone Size:6400; Vector Backbone:pEC-K-MBP; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22745249
Proper citation: RRID:Addgene_39317 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39280 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 7bp deletion (ATATAAA), 2nt mismatch at bp#4267-4268 and 7nt deletion at bp#4269-4275 when compared to EU493333.1. Does not effect function.
Proper citation: RRID:Addgene_39282 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39307 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39303 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39306 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This mutant does not bind microtubule tips.
Proper citation: RRID:Addgene_39300 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39302 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39301 Copy
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5438; Vector Backbone:pET26b(+); Vector Types:Bacterial Expression, Bacterial expression, bacterial expression of recombinant antibody fragments in the form of single chain Fvs (scFvs); Bacterial Resistance:Kanamycin
Defining Citation: PMID:17156422
Proper citation: RRID:Addgene_39264 Copy
Genetic Insert: RAJ31 system
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4860; Vector Backbone:pSTC1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39255 Copy
Genetic Insert: RAJ31 system (no terminator for the transRNA)
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4860; Vector Backbone:pSTC1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39257 Copy
Genetic Insert: RAJ22 system (no transRNA)
Vector Backbone Description: Backbone Marker:Jaramillo Lab; Backbone Size:4042; Vector Backbone:pSTC0; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22949707
Proper citation: RRID:Addgene_39252 Copy
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