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Species: Danio rerio
Genetic Insert: crhr2 pTAL scaffold 1A
Vector Backbone Description: Backbone Marker:Addgene plasmid 38142; Backbone Size:4651; Vector Backbone:RCIscript-GoldyTALEN; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23000899
Comments: Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.
Proper citation: RRID:Addgene_44236 Copy
Species: Danio rerio
Genetic Insert: crhr1 pTAL scaffold 1B
Vector Backbone Description: Backbone Marker:Addgene plasmid 38142; Backbone Size:4651; Vector Backbone:RCIscript-GoldyTALEN; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23000899
Comments: Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.
Proper citation: RRID:Addgene_44235 Copy
Species: Synthetic
Genetic Insert: Laconic (LACtate Optical Nano Indicator from CECs)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5418; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression, lactate biosensor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23469056
Comments: Laconic is a genetically-encoded biosensor that detects lactate in the physiological range. The sensor was based on LldR, a bacterial transcription regulator that consists of a lactate-binding/regulatory domain and a DNA-binding domain. Laconic consists of a N-terminal mTFP, followed by the LldR flanked by linkers, and finally a C-terminal Venus.
See Addgene plasmid# 46307: NLS-Laconic/pCMV-myc-nuc for a nuclear-targeted version of Laconic: http://www.addgene.org/46307/ .
Proper citation: RRID:Addgene_44238 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha 0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17576765
Comments: Dronpa was inserted between amino acid 92 and 93 using the KpnI and SpeI sites which were engineered between these sites.
Proper citation: RRID:Addgene_44193 Copy
Species: Homo sapiens
Genetic Insert: RK-GFP
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8766; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20517486
Proper citation: RRID:Addgene_44351 Copy
Species: Homo sapiens
Genetic Insert: NIPP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Proper citation: RRID:Addgene_44229 Copy
Species: Homo sapiens
Genetic Insert: PP1B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Comments: Please note that there is a silent mutation at bp#842 when compared to GenBank reference sequence NM_002709.2.
Proper citation: RRID:Addgene_44223 Copy
Species: Homo sapiens
Genetic Insert: PP-1G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Comments: Please note that there is a silent mutation at bp#784 when compared to GenBank reference sequence NM_002710.3.
Proper citation: RRID:Addgene_44225 Copy
Species: Xenopus laevis
Genetic Insert: XOPS-tdTOM
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8760; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20517486
Proper citation: RRID:Addgene_44347 Copy
Species: Homo sapiens
Genetic Insert: TAF10
Vector Backbone Description: Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503660
Proper citation: RRID:Addgene_44340 Copy
Species: Homo sapiens
Genetic Insert: TAF7
Vector Backbone Description: Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503660
Proper citation: RRID:Addgene_44338 Copy
Species: Synthetic
Genetic Insert: CBF1-Responsive-Element
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4540; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23617465
Proper citation: RRID:Addgene_44211 Copy
Species: Homo sapiens
Genetic Insert: RepoMan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16492807
Proper citation: RRID:Addgene_44213 Copy
Species: Mus musculus
Genetic Insert: TAF6
Vector Backbone Description: Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503660
Proper citation: RRID:Addgene_44337 Copy
Species: Homo sapiens
Genetic Insert: TAF5
Vector Backbone Description: Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23503660
Proper citation: RRID:Addgene_44336 Copy
Species: Yarrowia lipolytica
Genetic Insert: UAS1B28-Leum promoter
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21926196
Proper citation: RRID:Addgene_44327 Copy
Species: Drosophila melanogaster
Genetic Insert: Qin
Vector Backbone Description: Vector Backbone:pUASp-FMW; Vector Types:Fly transformation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099305
Proper citation: RRID:Addgene_44209 Copy
Species: Homo sapiens
Genetic Insert: human GPR56 C-terminal fragment with signal peptide
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:7500; Vector Backbone:Barcode; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_44201 Copy
Vector Backbone Description: Backbone Size:7500; Vector Backbone:Barcode; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_44200 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 1nt mismatch at bp#102 which may affect NheI site. Should not effect plasmid function.
Proper citation: RRID:Addgene_39295 Copy
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