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Species: Saccharomyces cerevisiae
Genetic Insert: Mevalonate decarboxylase (MDC)
Vector Backbone Description: Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30085399
Comments: codon-optimized synthetic gene for expression in E. coli
Proper citation: RRID:Addgene_108950 Copy
Species: Homo sapiens
Genetic Insert: Non-targeting sgRNA #3
Vector Backbone Description: Backbone Marker:Jonathan Weissman, Addgene #84833; Backbone Size:8200; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Proper citation: RRID:Addgene_109003 Copy
Species: Homo sapiens
Genetic Insert: ATL1-sgRNA #2
Vector Backbone Description: Backbone Marker:Jonathan Weissman, Addgene #84836; Backbone Size:8200; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Proper citation: RRID:Addgene_109006 Copy
Species: Homo sapiens
Genetic Insert: ATL1-sgRNA #5
Vector Backbone Description: Backbone Marker:Jonathan Weissman, Addgene #84837; Backbone Size:8200; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Proper citation: RRID:Addgene_109007 Copy
Species: Homo sapiens
Genetic Insert: ATL2-sgRNA #1
Vector Backbone Description: Backbone Marker:Jonathan Weissman, Addgene #84838; Backbone Size:8200; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Proper citation: RRID:Addgene_109008 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pBC SK; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109061 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109058 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_109059 Copy
Genetic Insert: PTSG_10057
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0011 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109072 Copy
Species: Synthetic
Genetic Insert: nanoluc
Vector Backbone Description: Vector Backbone:pDSB_0054; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0055 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109078 Copy
Species: Mus musculus
Genetic Insert: ANGPTL3
Vector Backbone Description: Backbone Size:4989; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29031715
Comments: 21bp deletion in the SV40 promoter sequence. May affect blasticidin selection.
Proper citation: RRID:Addgene_109111 Copy
Genetic Insert: PTSG_03784
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0044 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109070 Copy
Genetic Insert: PTSG_02256
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0043 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109068 Copy
Genetic Insert: PTSG_07215
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0102 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109101 Copy
Genetic Insert: PTSG_05705
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0027 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109069 Copy
Genetic Insert: PTSG_06009
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0100 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109103 Copy
Genetic Insert: 3'UTR EFL
Vector Backbone Description: Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0106 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109104 Copy
Genetic Insert: PTSG_06009
Vector Backbone Description: Vector Backbone:pDSB_0106; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0107 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109105 Copy
Species: Homo sapiens
Genetic Insert: human full-length CoREST, sequence-optimized for insect cells
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28218430
Proper citation: RRID:Addgene_109156 Copy
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