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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
 
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1+ mentions
RRID:Addgene_169168 MAP1LC3B Homo sapiens Ampicillin PMID:29343546 Internal construct reference: SMC-948 Backbone Marker:Merck; Backbone Size:5420; Vector Backbone:pETDuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-08 01:12:08 1
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
 
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RRID:Addgene_169161 14His-SUMO-nsp10-GGSGGS-nsp14 Synthetic Kanamycin PMID:34198326 Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimised for E. coli 2026-08-08 01:12:08 0
pH6-TEV-spTorA-GFP(C)
 
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RRID:Addgene_169041 H6-spTorA-GFP Escherichia coli and Aequorea victoria Ampicillin PMID:34499687 The spTorA-GFP coding sequence was recovered from p-spTorA-GFP-H6C, a plasmid originally reported as pTorA-GFP in Bageshwar & Musser 2007 J. Cell Biol., 179:87-99. Backbone Marker:Beckwith Lab; Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin The mut3 GFP variant with 5 additional mutations (Q80R, S147H, M153T, V163A, S202D). There are also 3 silent mutations at G65 (GGT instead of GGG), Q69 (CAA instead of CAG) and P75 (CCA instead of CCG). Internal NcoI site in GFP was deleted (CCATGG to CCCTGG). 2026-08-08 01:12:07 0
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)
 
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RRID:Addgene_169162 nsp10-6His-3xFlag Synthetic Ampicillin and Spectinomycin PMID:34198326 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)
 
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RRID:Addgene_169164 nsp14/nsp10-6His-3xFlag Synthetic Chloramphenicol and Ampicillin PMID:34198326 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG2ab (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pCOLADuet-1_nsp10 (SARS-CoV-2)
 
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RRID:Addgene_169158 nsp10 SARS-CoV-2 Kanamycin PMID:34198326 Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells. Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimised for E. coli 2026-08-08 01:12:08 0
pET11a_3xFlag-nsp14 (SARS-CoV-2)
 
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RRID:Addgene_169159 3xFlag-nsp5CS[VATLQ]-nsp14 SARS-CoV-2 Ampicillin PMID:34198326 We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells. Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon optimised for E. coli 2026-08-08 01:12:08 0
pET11a_3xFlag-nsp10 (SARS-CoV-2)
 
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RRID:Addgene_169157 3xFlag-nsp5CS[VRLQ]-nsp10 Synthetic Ampicillin PMID:34198326 We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells. Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon optimised for E. coli 2026-08-08 01:12:08 0
pAC-U63-tgRNA-Gal80
 
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RRID:Addgene_169030 Ampicillin PMID:33444322 For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/ Backbone Size:12746; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-08 01:12:08 0
pMAL724
 
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RRID:Addgene_169151 VP16-EL222 Erythrobacter litoralis Ampicillin PMID:34346207 Vector Backbone:pUC; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin A79Q 2026-08-08 01:12:08 0
pIB1884-ximD-PCPT
 
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RRID:Addgene_169109 XimD and PcPT Synthetic Apramycin PMID:32786357 Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-08 01:12:08 0
pET28a Gx-NL
 
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RRID:Addgene_169105 protein G - NanoLuc Synthetic Kanamycin PMID:31909607 Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ala 24 stop 2026-08-08 01:12:08 0
pET28a Gx-mNG-NL
 
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RRID:Addgene_169106 protein G - mNeonGreen - NanoLuc Synthetic Kanamycin PMID:31909607 Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ala 24 stop 2026-08-08 01:12:08 0
pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)
 
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RRID:Addgene_169177 3xFlag-His-nsp5CS-nsp9 SARS-CoV-2 Ampicillin PMID:34198327 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pBIG1b_nsp7 (SARS-CoV-2)
 
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RRID:Addgene_169178 nsp7 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pBIG1c_nsp8 (SARS-CoV-2)
 
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RRID:Addgene_169179 nsp8 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)
 
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RRID:Addgene_169181 nsp7-Linker-nsp8 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-08 01:12:08 0
pNVLTv2-pvdIJD-Ptrc-base
 
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RRID:Addgene_169243 lacI Escherichia coli Kanamycin PMID:34175462 Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:12:09 0
pNVLTv2-pvdIJD-Prha-base
 
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RRID:Addgene_169244 rhaRS Escherichia coli Kanamycin PMID:34175462 Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function. Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:12:09 0
pNVLTv2
 
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RRID:Addgene_169245 Kanamycin PMID:34175462 Please note that the P10S mutation in the BLMT family bleomycin binding protein does not affect overall plasmid function. Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-08 01:12:09 0

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