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Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Backbone Marker:Merck; Backbone Size:5420; Vector Backbone:pETDuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29343546
Comments: Internal construct reference: SMC-948
Proper citation: RRID:Addgene_169168 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GGSGGS-nsp14
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Proper citation: RRID:Addgene_169161 Copy
Species: Escherichia coli and Aequorea victoria
Genetic Insert: H6-spTorA-GFP
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34499687
Comments: The spTorA-GFP coding sequence was recovered from p-spTorA-GFP-H6C, a plasmid originally reported as pTorA-GFP in Bageshwar & Musser 2007 J. Cell Biol., 179:87-99.
Proper citation: RRID:Addgene_169041 Copy
Species: Synthetic
Genetic Insert: nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169162 Copy
Species: Synthetic
Genetic Insert: nsp14/nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG2ab (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169164 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10
Vector Backbone Description: Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Comments: Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.
Proper citation: RRID:Addgene_169158 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-nsp5CS[VATLQ]-nsp14
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169159 Copy
Species: Synthetic
Genetic Insert: 3xFlag-nsp5CS[VRLQ]-nsp10
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169157 Copy
Vector Backbone Description: Backbone Size:12746; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33444322
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_169030 Copy
Species: Erythrobacter litoralis
Genetic Insert: VP16-EL222
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346207
Proper citation: RRID:Addgene_169151 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:32786357
Proper citation: RRID:Addgene_169109 Copy
Species: Synthetic
Genetic Insert: protein G - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169105 Copy
Species: Synthetic
Genetic Insert: protein G - mNeonGreen - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169106 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-His-nsp5CS-nsp9
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198327
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169177 Copy
Species: Synthetic
Genetic Insert: nsp7
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169178 Copy
Species: Synthetic
Genetic Insert: nsp8
Vector Backbone Description: Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169179 Copy
Species: Synthetic
Genetic Insert: nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169181 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169243 Copy
Species: Escherichia coli
Genetic Insert: rhaRS
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function.
Proper citation: RRID:Addgene_169244 Copy
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the P10S mutation in the BLMT family bleomycin binding protein does not affect overall plasmid function.
Proper citation: RRID:Addgene_169245 Copy
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