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Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29074583
Comments: LAMP1-RFP was cloned from Plasmid #34611
Proper citation: RRID:Addgene_102931 Copy
Species: Rattus norvegicus
Genetic Insert: KIF1A-shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:5415; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27658622
Comments: The pmTurquoise KIF1A shRNA vector was constructed by restriction site removal of eGFP from the commercially available pSuper vector (Oligoengine) and insertion of the cDNA coding for pmTurquoise (Addgene plasmid #36202).
The reference for the pSuper Vector (Oligoengine) is T.R. Brummelkamp, R. Bernards, and R Agami, Science 296, 550 (2002).
Proper citation: RRID:Addgene_102849 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Addgene Plasmid #160210).
Proper citation: RRID:Addgene_160211 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Addgene Plasmid # 160208).
Proper citation: RRID:Addgene_160209 Copy
Species: Rattus norvegicus
Genetic Insert: DHFR-mNeon-GluA1
Vector Backbone Description: Backbone Size:4872; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34241635
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.02.278770v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_173009 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES-GFP
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971908
Proper citation: RRID:Addgene_17301 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17331 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged2
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17332 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6100; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The backbone of this plasmid appears to be approximately 600bp smaller than is reported. The depositing scientist is aware of this difference and reports that the plasmid functions as it should.
Proper citation: RRID:Addgene_17336 Copy
Species: Rattus norvegicus
Genetic Insert: ZAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCDNA4 TO2 myc HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215647
Comments: The NZAP fragment was excised from pBabe-NZAP-Zeo with EcoRI-NotI and cloned into pCDNA4/TO2-myc-HisB to generate a myc-tagged
NZAP. The C-terminal portion of ZAP was cloned from a Rat2 cell cDNA library by PCR using sense primer CZAP-SP (5’-GAGCTCTCTGGGCTTAACC-3’) and antisense primer CZAP-AP (5’-
ATATAGGCGGCCGCCCTCTGGACCTCTTCTCTTC-3’). The sense primer lies
upstream from an internal NheI site in NZAP; the antisense primer introduces a NotI site immediately downstream from the coding sequence to facilitate its cloning into the myc-tagged expression vector.
Proper citation: RRID:Addgene_17381 Copy
Species: Rattus norvegicus
Genetic Insert: NZAP
Vector Backbone Description: Backbone Marker:Goff Lab; Backbone Size:0; Vector Backbone:pBabe-HAZ (modified pBabe vector); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12215647
Proper citation: RRID:Addgene_17382 Copy
Species: Rattus norvegicus
Genetic Insert: Gai3-YFP
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27072656
Proper citation: RRID:Addgene_171375 Copy
Species: Rattus norvegicus
Genetic Insert: GBAi
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pLIC-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29133411
Proper citation: RRID:Addgene_171756 Copy
Species: Rattus norvegicus
Genetic Insert: GBAi
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pLIC-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29133411
Proper citation: RRID:Addgene_171754 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES
Vector Backbone Description: Backbone Size:33400; Vector Backbone:Adenoviral Recombinant (pAdEasy); Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17420246
Comments: AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_17286 Copy
Species: Rattus norvegicus
Genetic Insert: PV-DsRed-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pcDNA5/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17420246
Proper citation: RRID:Addgene_16341 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES-DsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pcDNA5/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17420246
Comments: Please note- this plasmid may contain the NES N-terminal to the PV insert, not C-terminal as the plasmid name suggests. Also, there is a Myc tag at the C-terminus of the DsRed.
Proper citation: RRID:Addgene_16339 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences. An additional C-terminal mutation, H579Y, is present. These mutations are not believed to affect function.
Proper citation: RRID:Addgene_16379 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences and are therefore not believed to affect function.
Proper citation: RRID:Addgene_16378 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_16380 Copy
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