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Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126472 Copy
Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126473 Copy
Species: Drosophila melanogaster
Genetic Insert: DmelOrco
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and PvuI-HF and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other. Use the EcoRI restriction site for best functional expression on the MCS2. Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126476 Copy
Species: Drosophila melanogaster
Genetic Insert: d-Dispatched1
Vector Backbone Description: Vector Backbone:pUAS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29359685
Proper citation: RRID:Addgene_126412 Copy
Species: Drosophila melanogaster
Genetic Insert: Or47a
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126478 Copy
Species: Drosophila melanogaster
Genetic Insert: Or56a
Vector Backbone Description: Backbone Size:4111; Vector Backbone:pCMV-BI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31504297
Comments: Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
Proper citation: RRID:Addgene_126479 Copy
Species: Drosophila melanogaster
Genetic Insert: U6:3-gRNA
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite:
Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111
In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Proper citation: RRID:Addgene_123366 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Rae1
Vector Backbone Description: Backbone Size:2563; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28554770
Proper citation: RRID:Addgene_124240 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Actin 5C
Vector Backbone Description: Backbone Size:2553; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124242 Copy
Species: Drosophila melanogaster
Genetic Insert: wg gRNA
Vector Backbone Description: Backbone Size:7473; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078888
Proper citation: RRID:Addgene_125006 Copy
Species: Drosophila melanogaster
Genetic Insert: dorsal RHR in pB1H2
Vector Backbone Description: Backbone Size:4093; Vector Backbone:pB1H2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16041365
Proper citation: RRID:Addgene_12613 Copy
Species: Drosophila melanogaster
Genetic Insert: LN-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:LamdaN entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128012 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
Species: Drosophila melanogaster
Genetic Insert: Dm-let-7
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Sequence extracted from NCBI: NR_001664.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145844 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP2_457-791
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145849 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1_1-326
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145846 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP2_1-457
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145847 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1_1-326
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145853 Copy
Species: Drosophila melanogaster
Genetic Insert: DmAUB
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145851 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1_1-273
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_145852 Copy
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