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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCLXSN-Tax Resource Report Resource Website 1+ mentions |
RRID:Addgene_44038 | 5'UTR-Tax-3'UTR | Homo sapiens | Ampicillin | PMID:11553609 | Transfer of BamHI-BamHI fragment covering 5'UTR-Tax-3'UTR from pCMV4-Tax (from Dr. Warner Greene) to the BamHI site of pCLXSN (from Dr. Verma Inder). | Backbone Marker:Dr. Inder Verma; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:12 | 1 | |
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pCoofy3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_43983 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. Note that the 6x His GST double tag tends to increase the formation of insoluble protein aggregates. | Backbone Marker:Novagen, EMBL; Backbone Size:6406; Vector Backbone:pET, pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 3 | |||
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pCoofy18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_43975 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. Note that depending on the protein, the 10x His tag can reduce or abolish expression. | Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 2 | |||
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pCoofy7 Resource Report Resource Website |
RRID:Addgene_43976 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen, EMBL; Backbone Size:5752; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:12 | 0 | |||
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pCoofy12 Resource Report Resource Website |
RRID:Addgene_43977 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:5809; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 0 | |||
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pCoofy21 Resource Report Resource Website |
RRID:Addgene_43979 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:5842; Vector Backbone:pET, pCoofy12; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:12 | 0 | |||
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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_44027 | mHIF-2α (P405A/P530V/N851A) | Mus musculus | Ampicillin | PMID:17804822 | This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α. Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. This plasmid has an A94V polymorphism that does not affect protein function. Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | P405A, P530V, N851A | 2026-08-15 01:15:11 | 7 |
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pGPD2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_43972 | Ampicillin | PMID:21586584 | Backbone Size:5500; Vector Backbone:p416-GPD; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 6 | ||||
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pCoofy1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_43974 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen, EMBL; Backbone Size:5737; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 4 | |||
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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A) Resource Report Resource Website 10+ mentions |
RRID:Addgene_44028 | mHIF-1α (P402A/P577A/N813A) | Mus musculus | Ampicillin | PMID:17804822 | This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-1α. Full-length murine HIF-1α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-1α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 402, Proline 577 and Asparagine 813 to alanines. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. Alternate plasmid names: HIF-1α triple mutant (TM); pcDNA3 HIF-1α TM | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | P402A, P577A, N813A | 2026-08-15 01:15:12 | 16 |
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pCT2-HRP Resource Report Resource Website |
RRID:Addgene_44021 | HRP | Synthetic | Ampicillin | PMID:17961829 | Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 0 | ||
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pFGC5941-PacI Resource Report Resource Website |
RRID:Addgene_44020 | Kanamycin | PMID:23098881 | Backbone Marker:http://www.arabidopsis.org/servlet/TairObject?id=500300075&type=vector; Backbone Size:11405; Vector Backbone:pFGC5941; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:12 | 0 | ||||
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pENTR puroR R4-R3 Resource Report Resource Website |
RRID:Addgene_43965 | puroR | Kanamycin | PMID:23637962 | Backbone Marker:Invitrogen; Backbone Size:2670; Vector Backbone:pDONR221-P4rP3r; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 0 | |||
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pENTR pA L3-L2 Resource Report Resource Website |
RRID:Addgene_43966 | pA | Kanamycin | PMID:23637962 | Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221-P3P2; Vector Types:Multisite Gateway ENTR clone; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 0 | |||
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N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS Resource Report Resource Website 1+ mentions |
RRID:Addgene_43969 | Cbx5 | Mus musculus | Ampicillin | PMID:22704655 | Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | N-term chromo domain removed | 2026-08-15 01:15:11 | 1 | |
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N114 nLV EF-1a-Gal4-VP16-PGK-Puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_44014 | Gal4/DBD (1-147) | Saccharomyces cerevisiae | Ampicillin | PMID:22704655 | Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 4 | ||
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N205 nLV EF-1a-ZFHD1-link-FKBP-HA Resource Report Resource Website 1+ mentions |
RRID:Addgene_44017 | FKBP | Homo sapiens | Ampicillin | PMID:22704655 | Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 2 | ||
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pCoofy33 Resource Report Resource Website |
RRID:Addgene_44001 | Kanamycin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:5827; Vector Backbone:pET, pCoofy18; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:11 | 0 | |||
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pCoofy11 Resource Report Resource Website |
RRID:Addgene_44002 | Ampicillin | PMID:23410102 | Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:4164; Vector Backbone:pIEX1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 0 | |||
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p3s-Cas9HC Resource Report Resource Website 10+ mentions |
RRID:Addgene_43945 | Cas9 | S. pyogenes | Ampicillin | PMID:23360966 | For more information on Kim Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kim/ | Backbone Marker:invitrogen; Backbone Size:3200; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:11 | 38 |
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