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Species: Homo sapiens
Genetic Insert: 5'UTR-Tax-3'UTR
Vector Backbone Description: Backbone Marker:Dr. Inder Verma; Vector Backbone:pCLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11553609
Comments: Transfer of BamHI-BamHI fragment covering 5'UTR-Tax-3'UTR from pCMV4-Tax (from Dr. Warner Greene) to the BamHI site of pCLXSN (from Dr. Verma Inder).
Proper citation: RRID:Addgene_44038 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6406; Vector Backbone:pET, pETM33; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that the 6x His GST double tag tends to increase the formation of insoluble protein aggregates.
Proper citation: RRID:Addgene_43983 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Note that depending on the protein, the 10x His tag can reduce or abolish expression.
Proper citation: RRID:Addgene_43975 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5752; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43976 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5809; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43977 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5842; Vector Backbone:pET, pCoofy12; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43979 Copy
Species: Mus musculus
Genetic Insert: mHIF-2α (P405A/P530V/N851A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17804822
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α.
Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
This plasmid has an A94V polymorphism that does not affect protein function.
Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM
Proper citation: RRID:Addgene_44027 Copy
Vector Backbone Description: Backbone Size:5500; Vector Backbone:p416-GPD; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21586584
Proper citation: RRID:Addgene_43972 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5737; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43974 Copy
Species: Mus musculus
Genetic Insert: mHIF-1α (P402A/P577A/N813A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17804822
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-1α.
Full-length murine HIF-1α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.
A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-1α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 402, Proline 577 and Asparagine 813 to alanines. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.
Alternate plasmid names: HIF-1α triple mutant (TM); pcDNA3 HIF-1α TM
Proper citation: RRID:Addgene_44028 Copy
Species: Synthetic
Genetic Insert: HRP
Vector Backbone Description: Backbone Size:6142; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17961829
Proper citation: RRID:Addgene_44021 Copy
Vector Backbone Description: Backbone Marker:http://www.arabidopsis.org/servlet/TairObject?id=500300075&type=vector; Backbone Size:11405; Vector Backbone:pFGC5941; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_44020 Copy
Genetic Insert: puroR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2670; Vector Backbone:pDONR221-P4rP3r; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43965 Copy
Genetic Insert: pA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221-P3P2; Vector Types:Multisite Gateway ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43966 Copy
Species: Mus musculus
Genetic Insert: Cbx5
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_43969 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4/DBD (1-147)
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44014 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44017 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5827; Vector Backbone:pET, pCoofy18; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_44001 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4164; Vector Backbone:pIEX1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_44002 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3200; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23360966
Comments: For more information on Kim Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kim/
Proper citation: RRID:Addgene_43945 Copy
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