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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAAV-CAG-eLACCO1.1 Resource Report Resource Website |
RRID:Addgene_168789 | eLACCO1.1 | Ampicillin | PMID:34873165 | Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint. | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||
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pGem-PgpdA-Cre-TtrpC Resource Report Resource Website |
RRID:Addgene_168784 | Cre | Synthetic | Ampicillin | PMID:35168324 | For transient Cre recombinase expression in Aspergillus nidulans, we created the helper vector pGem-PgpdA-Cre-TtrpC unable to replicate in filamentous fungi. The helper vector encodes a cassette for constitutive expression of Cre under the promoter gpdA (PgpdA) and the terminator trpC . As the recipient strain A. nidulans LP1 carries nkuA∆, which minimises random integration events, the helper vector presumably would be lost during fungal growth | Backbone Marker:Promega; Backbone Size:2812; Vector Backbone:pGem; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | |
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pGem-LP1-loxP-bar-lox2272-71 Resource Report Resource Website |
RRID:Addgene_168785 | bar | Synthetic | Ampicillin | PMID:35168324 | Vector used to create Aspergillus nidulans parental strains with LP1 by polyethylene glycol (PEG)-calcium-based transformation with NotI linearized vector pGemLP1-loxP-bar-Lox2272-71 containing 1 kb homology regions at 5′ and 3′ of the floxed bar to facilitate homologous recombination in A. nidulans LO8030 ∆St locus. Colonies are selected for resistance to glufosinate extracted from Basta. | Backbone Size:3033; Vector Backbone:pGEM; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | |
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ZP44 Wnt8 5' orf2 Resource Report Resource Website |
RRID:Addgene_16898 | wnt8 5'orf2 | Danio rerio | Ampicillin | 588 bp PCR product ligated into BamHI and EcoRI cut pSK+. For probe: cut with BamHI, use T7 RNA polymerase. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||
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ZP45 Prickle-1 Resource Report Resource Website |
RRID:Addgene_16897 | Prickle-1 | Danio rerio | Ampicillin | PMID:12699626 | Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker. To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | |
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pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66 Resource Report Resource Website 1+ mentions |
RRID:Addgene_168787 | Ampicillin | PMID:35168324 | pRecomb-loxp-mcherry-4Gcloningsite-lox71-2272-66 (donor vector 2) is shuttle vector that supports optional transformation-associated recombination cloning in Saccharomyces cerevisiae. Donor vector 2 also contains within the floxed region four cloning sites for the expression of biosynthetic genes under strong alcohol inducible promoters, derived from the vector pYFAC-CH2. As the four-promoter multiple cloning site is flanked by EcoRI sites, donor vector 2 can also be used for cloning genomic fragments containing a BGC. | Vector Backbone:pRecomb-loxp-mCherry-4Gcloningsite-lox2272-66; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 1 | |||
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ZP46 Prickle-2 Resource Report Resource Website |
RRID:Addgene_16896 | Prickle-2 | Danio rerio | Ampicillin | PMID:12699626 | Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+. To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Size:0; Vector Backbone:CS2P+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | |
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LifeAct-TagBFP-DiB-RM Resource Report Resource Website |
RRID:Addgene_168782 | LifeAct-TagBFP-DiB-RM | Synthetic | Kanamycin | PMID:34748541 | Backbone Size:3948; Vector Backbone:pDendra2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-08 01:12:07 | 0 | ||
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pBK125 Resource Report Resource Website |
RRID:Addgene_1688 | Ampicillin | See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pBK125.1, Ligation number pBK125. | Backbone Size:4543; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||||
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pJKL464 Resource Report Resource Website |
RRID:Addgene_1689 | Ampicillin | See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pKL464.1, Ligation number pJKL464. | Backbone Size:5850; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||||
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XE7 Xwnt-8 Resource Report Resource Website |
RRID:Addgene_16869 | Xwnt-8 RNA | Xenopus laevis | Ampicillin | PMID:1879349 | Insert: EcoR1 insert blunted into Bgl II blunt of SP64T. Transcription: linearize with Bam H1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian on date of 12/90. Citation: Christian et al., Development 111, 1045 (1991) | Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:06 | 0 | |
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pYFAC-pyroA Resource Report Resource Website |
RRID:Addgene_168976 | Ampicillin | PMID:30809363 | A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5 | Backbone Size:14581; Vector Backbone:pYFAC-pyroA; Vector Types:Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | |||
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pYFAC-CH1 Resource Report Resource Website |
RRID:Addgene_168977 | Ampicillin | PMID:30809363 | Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:Synthetic Biology, Heterologous expression in filamentous fungi.; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||||
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pYFAC-CH2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_168978 | Ampicillin | PMID:30809363 | A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5 | Vector Backbone:pYFAC-pyrG; Vector Types:Synthetic Biology, Heterologous expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 2 | |||
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XE10 Xwnt-8 CS2+ Resource Report Resource Website |
RRID:Addgene_16865 | Xwnt-8 | Xenopus laevis | Ampicillin | EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+; Sense RNA cut with ASP718 and transcribe with SP6. Constructed by Jeff Brown, unpublished. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:06 | 0 | ||
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TagBFP-DiB2-splitN1–125 Resource Report Resource Website |
RRID:Addgene_168973 | TagBFP - DiB2-split N-frag | Synthetic | Ampicillin | PMID:32632329 | Backbone Size:5296; Vector Backbone:pICH47732; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 0 | ||
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XE12 Xwnt-11 Resource Report Resource Website |
RRID:Addgene_16861 | Xwnt-11 | Xenopus laevis | Ampicillin | PMID:7739543 | Insert: full length coding seq only, by PCR of lambda clones isolated by Purcell. Insert size: 1 Kb. Transciption: linearize with Sal1, Xba1, or BamH1 and transcribe with T7. Plasmid History: Constructed by Susan Purcell on date of 5/94. Citation: Du et al., Molec. Cell Bio., 15, 2625 (1995) Ku and Melton, Development, 119, 1161 (1993) | Backbone Size:0; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:06 | 0 | |
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pYFAC-CH3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_168979 | Ampicillin | PMID:30809363 | A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5 | Backbone Size:15497; Vector Backbone:pYFAC-riboB; Vector Types:Synthetic Biology, Heterologous expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:07 | 1 | |||
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XE62 Xwnt-4 myc-SP64T Resource Report Resource Website |
RRID:Addgene_16877 | Xwnt-4 myc | Xenopus laevis | Ampicillin | See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus. Transcription: linearize with Sal1 and transcribe with SP6. Citation: L. Lynn McGrew, unpublished. | Backbone Size:2999; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:06 | 0 | ||
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XE63 Xwnt-4 myc-CSKA Resource Report Resource Website |
RRID:Addgene_16876 | Xwnt-4myc | Xenopus laevis | Ampicillin | See clone XE2 for details and XE62 for myc tag, and V4 for vector details. Notes for Use: Citation: L. Lynn McGrew, unpublished. | Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-08 01:12:06 | 0 |
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