Plasmids   Select Another Resource Report Type

http://www.addgene.org/168789

Genetic Insert: eLACCO1.1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34873165
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_168789 Copy   

•   Source: Addgene

http://www.addgene.org/168784

Species: Synthetic
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2812; Vector Backbone:pGem; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35168324
Comments: For transient Cre recombinase expression in Aspergillus nidulans, we created the helper vector pGem-PgpdA-Cre-TtrpC unable to replicate in filamentous fungi. The helper vector encodes a cassette for constitutive expression of Cre under the promoter gpdA (PgpdA) and the terminator trpC . As the recipient strain A. nidulans LP1 carries nkuA∆, which minimises random integration events, the helper vector presumably would be lost during fungal growth

Proper citation: RRID:Addgene_168784 Copy   

•   Source: Addgene

http://www.addgene.org/168785

Species: Synthetic
Genetic Insert: bar
Vector Backbone Description: Backbone Size:3033; Vector Backbone:pGEM; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35168324
Comments: Vector used to create Aspergillus nidulans parental strains with LP1 by polyethylene glycol (PEG)-calcium-based transformation with NotI linearized vector pGemLP1-loxP-bar-Lox2272-71 containing 1 kb homology regions at 5′ and 3′ of the floxed bar to facilitate homologous recombination in A. nidulans LO8030 ∆St locus. Colonies are selected for resistance to glufosinate extracted from Basta.

Proper citation: RRID:Addgene_168785 Copy   

•   Source: Addgene

http://www.addgene.org/16898

Species: Danio rerio
Genetic Insert: wnt8 5'orf2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: 588 bp PCR product ligated into BamHI and EcoRI cut pSK+. For probe: cut with BamHI, use T7 RNA polymerase.

Proper citation: RRID:Addgene_16898 Copy   

•   Source: Addgene

http://www.addgene.org/16897

Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker. To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_16897 Copy   

•   Source: Addgene

http://www.addgene.org/168787

Vector Backbone Description: Vector Backbone:pRecomb-loxp-mCherry-4Gcloningsite-lox2272-66; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35168324
Comments: pRecomb-loxp-mcherry-4Gcloningsite-lox71-2272-66 (donor vector 2) is shuttle vector that supports optional transformation-associated recombination cloning in Saccharomyces cerevisiae. Donor vector 2 also contains within the floxed region four cloning sites for the expression of biosynthetic genes under strong alcohol inducible promoters, derived from the vector pYFAC-CH2. As the four-promoter multiple cloning site is flanked by EcoRI sites, donor vector 2 can also be used for cloning genomic fragments containing a BGC.

Proper citation: RRID:Addgene_168787 Copy   

•   Source: Addgene

http://www.addgene.org/16896

Species: Danio rerio
Genetic Insert: Prickle-2
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2P+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+. To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_16896 Copy   

•   Source: Addgene

http://www.addgene.org/168782

Species: Synthetic
Genetic Insert: LifeAct-TagBFP-DiB-RM
Vector Backbone Description: Backbone Size:3948; Vector Backbone:pDendra2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34748541

Proper citation: RRID:Addgene_168782 Copy   

•   Source: Addgene

http://www.addgene.org/1688

Vector Backbone Description: Backbone Size:4543; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pBK125.1, Ligation number pBK125.

Proper citation: RRID:Addgene_1688 Copy   

•   Source: Addgene

http://www.addgene.org/1689

Vector Backbone Description: Backbone Size:5850; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pKL464.1, Ligation number pJKL464.

Proper citation: RRID:Addgene_1689 Copy   

•   Source: Addgene

http://www.addgene.org/16869

Species: Xenopus laevis
Genetic Insert: Xwnt-8 RNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1879349
Comments: Insert: EcoR1 insert blunted into Bgl II blunt of SP64T. Transcription: linearize with Bam H1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian on date of 12/90. Citation: Christian et al., Development 111, 1045 (1991)

Proper citation: RRID:Addgene_16869 Copy   

•   Source: Addgene

http://www.addgene.org/168976

Vector Backbone Description: Backbone Size:14581; Vector Backbone:pYFAC-pyroA; Vector Types:Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30809363
Comments: A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5

Proper citation: RRID:Addgene_168976 Copy   

•   Source: Addgene

http://www.addgene.org/168977

Vector Backbone Description: Backbone Marker:doi:10.1038/nchembio.1366; Backbone Size:14245; Vector Backbone:pKW20088; Vector Types:Synthetic Biology, Heterologous expression in filamentous fungi.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30809363

Proper citation: RRID:Addgene_168977 Copy   

•   Source: Addgene

http://www.addgene.org/168978

Vector Backbone Description: Vector Backbone:pYFAC-pyrG; Vector Types:Synthetic Biology, Heterologous expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30809363
Comments: A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5

Proper citation: RRID:Addgene_168978 Copy   

•   Source: Addgene

http://www.addgene.org/16865

Species: Xenopus laevis
Genetic Insert: Xwnt-8
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+; Sense RNA cut with ASP718 and transcribe with SP6. Constructed by Jeff Brown, unpublished.

Proper citation: RRID:Addgene_16865 Copy   

•   Source: Addgene

http://www.addgene.org/168973

Species: Synthetic
Genetic Insert: TagBFP - DiB2-split N-frag
Vector Backbone Description: Backbone Size:5296; Vector Backbone:pICH47732; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632329

Proper citation: RRID:Addgene_168973 Copy   

•   Source: Addgene

http://www.addgene.org/16861

Species: Xenopus laevis
Genetic Insert: Xwnt-11
Vector Backbone Description: Backbone Size:0; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7739543
Comments: Insert: full length coding seq only, by PCR of lambda clones isolated by Purcell. Insert size: 1 Kb. Transciption: linearize with Sal1, Xba1, or BamH1 and transcribe with T7. Plasmid History: Constructed by Susan Purcell on date of 5/94. Citation: Du et al., Molec. Cell Bio., 15, 2625 (1995) Ku and Melton, Development, 119, 1161 (1993)

Proper citation: RRID:Addgene_16861 Copy   

•   Source: Addgene

http://www.addgene.org/168979

Vector Backbone Description: Backbone Size:15497; Vector Backbone:pYFAC-riboB; Vector Types:Synthetic Biology, Heterologous expression in Aspergillus nidulans and related filamentous fungi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30809363
Comments: A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5

Proper citation: RRID:Addgene_168979 Copy   

•   Source: Addgene

http://www.addgene.org/16877

Species: Xenopus laevis
Genetic Insert: Xwnt-4 myc
Vector Backbone Description: Backbone Size:2999; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus. Transcription: linearize with Sal1 and transcribe with SP6. Citation: L. Lynn McGrew, unpublished.

Proper citation: RRID:Addgene_16877 Copy   

•   Source: Addgene

http://www.addgene.org/16876

Species: Xenopus laevis
Genetic Insert: Xwnt-4myc
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: See clone XE2 for details and XE62 for myc tag, and V4 for vector details. Notes for Use: Citation: L. Lynn McGrew, unpublished.

Proper citation: RRID:Addgene_16876 Copy   

•   Source: Addgene