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Species: Oryza sativa
Genetic Insert: OsaCCH
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168411 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP31
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168412 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP56
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168413 Copy
Genetic Insert: N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168496 Copy
Genetic Insert: mouse PKA regulatory subunit IIα, untagged
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168492 Copy
Species: Oryza sativa
Genetic Insert: OsaHPP07
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168418 Copy
Genetic Insert: the K8,9E mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168487 Copy
Species: Oryza sativa
Genetic Insert: LOC_ Os04g32830
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168400 Copy
Genetic Insert: the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168488 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP18
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168402 Copy
Genetic Insert: the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168484 Copy
Genetic Insert: the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33876760
Proper citation: RRID:Addgene_168482 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP01
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168408 Copy
Species: Homo sapiens
Genetic Insert: CTCF
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTCF in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EDLYFQS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Proper citation: RRID:Addgene_168799 Copy
Species: Homo sapiens
Genetic Insert: gRNA library targeting human genes
Vector Backbone Description: Backbone Marker:Kosuke Yusa (Addgene #67974); Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34096786
Proper citation: RRID:Addgene_168791 Copy
Species: Danio rerio
Genetic Insert: sp5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11641225
Comments: Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.
Proper citation: RRID:Addgene_16884 Copy
Species: Danio rerio
Genetic Insert: 23f17
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis.
Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions.
For antisense: EcoRI, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16883 Copy
Species: Danio rerio
Genetic Insert: axin2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15797017
Comments: Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis.
Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling.
For antisense: Asp718, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
Proper citation: RRID:Addgene_16882 Copy
Genetic Insert: eLacco1.1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34873165
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168790 Copy
Genetic Insert: eLACCO1.1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34873165
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168788 Copy
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