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Species: Homo sapiens
Genetic Insert: Microtubule-associated protein tau 10-12 V337M
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36533443
Proper citation: RRID:Addgene_194165 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated protein tau 7-12 WT
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36533443
Proper citation: RRID:Addgene_194166 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36533443
Proper citation: RRID:Addgene_194163 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated protein tau 10-12 WT
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36533443
Proper citation: RRID:Addgene_194164 Copy
Species: Homo sapiens
Genetic Insert: CEACAM8
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_156730 Copy
Species: Homo sapiens
Genetic Insert: NEK10
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189874 Copy
Genetic Insert: aadA.co-TaadA-PrbcL-eYFP.co
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4636; Vector Backbone:pJMH020; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39392130
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498297v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_186641 Copy
Species: Homo sapiens
Genetic Insert: pEF1a-eGFP-T2A-BSD-bGHpA
Vector Backbone Description: Backbone Marker:The Center for Synthetic Regulatory Genetics at NYU Langone Health; Backbone Size:11564; Vector Backbone:pLM1110_LEU2 with a HSV-ΔTK counterselectable marker cassette; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology, Recombinase-mediated cassette exchange (RMCE); Bacterial Resistance:Kanamycin
Defining Citation: PMID:33649239
Comments: Please note plasmid contains an IS1 element. Depositor confirms this is not a concern for function.
Proper citation: RRID:Addgene_184751 Copy
Species: Mus musculus
Genetic Insert: Halo-CAMSAP3
Vector Backbone Description: Vector Backbone:P_actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36332023
Proper citation: RRID:Addgene_191329 Copy
Species: Homo sapiens
Genetic Insert: SEMA6B
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_156918 Copy
Species: Homo sapiens
Genetic Insert: MERTK
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_156878 Copy
Species: N/A
Genetic Insert: Engineered helicase PcrA_M6
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5676; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36274095
Proper citation: RRID:Addgene_194156 Copy
Species: Drosophila melanogaster
Genetic Insert: DmCaf40_25-291-Y139DG146W
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29255063
Comments: ORF extracted from NCBI: NM_167675.1. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148736 Copy
Genetic Insert: T2A-Cas9
Vector Backbone Description: Vector Backbone:pACGCB(2.1)-Gal4; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33782117
Proper citation: RRID:Addgene_194768 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGe1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16815998
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146688 Copy
Genetic Insert: Fos-CreERT2
Vector Backbone Description: Vector Backbone:Mammalian Gene Expression AAV Vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36564546
Proper citation: RRID:Addgene_194643 Copy
Genetic Insert: TRE3G-tdTomato
Vector Backbone Description: Vector Backbone:Mammalian Gene Expression AAV Vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36564546
Proper citation: RRID:Addgene_194644 Copy
Species: Homo sapiens
Genetic Insert: ISLR2_Ig1-3
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157619 Copy
Species: Homo sapiens
Genetic Insert: ISLR2_LRR1-7
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157618 Copy
Species: Synthetic
Genetic Insert: synER Cassette
Vector Backbone Description: Backbone Size:1870; Vector Backbone:pYKT095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194484 Copy
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