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Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168220 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168188 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168189 Copy
Species: Homo sapiens
Genetic Insert: HA-nLag6-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168191 Copy
Species: Homo sapiens
Genetic Insert: flag-Chibby
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pCS3+FLT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16824 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Chibby
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16822 Copy
Species: Synthetic
Genetic Insert: Leucine Zipper + C-fragment of wtBlc
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168256 Copy
Species: E. coli
Genetic Insert: C-fragment of wtBlc
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168257 Copy
Genetic Insert: PBD GFP
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168252 Copy
Species: Danio rerio
Genetic Insert: cdk2 sgRNAs
Vector Backbone Description: Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168250 Copy
Species: Drosophila melanogaster
Genetic Insert: armadillo
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2261639
Comments: Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
Proper citation: RRID:Addgene_16829 Copy
Genetic Insert: RacFRET
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Comments: Reference for the RacFRET (Raichu-Rac1) element:
D. L. Graham, P. N. Lowe, and P. A. Chalk. A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein. Anal.Biochem. 296:208-217, 2001.
Proper citation: RRID:Addgene_168247 Copy
Genetic Insert: Rac(guide resistant)-CA
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168244 Copy
Species: Setaria italica
Genetic Insert: Si514722593
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168316 Copy
Species: Setaria italica
Genetic Insert: Si514728335
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168317 Copy
Species: Setaria italica
Genetic Insert: Si944233294
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168311 Copy
Species: Oryza sativa
Genetic Insert: OsaHIP26
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168393 Copy
Species: Setaria italica
Genetic Insert: Si514742730
Vector Backbone Description: Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34758051
Proper citation: RRID:Addgene_168319 Copy
Species: Synthetic
Genetic Insert: HyPer7.2DAAO-mito
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32590330
Proper citation: RRID:Addgene_168304 Copy
Species: Synthetic
Genetic Insert: SaKKH-EGFP-U6-sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398178
Proper citation: RRID:Addgene_168305 Copy
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