Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26775 Copy
Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8688416
Comments: There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
Proper citation: RRID:Addgene_26774 Copy
Genetic Insert: EF1a-GFP-IRES-Puro
Vector Backbone Description: Backbone Size:7063; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Allows for puromycin selection of stable integration of GFP expression cassette. With modification, this vector enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting.
Proper citation: RRID:Addgene_26777 Copy
Genetic Insert: GFP*35
Vector Backbone Description: Backbone Size:7063; Vector Backbone:pEGIP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Proper citation: RRID:Addgene_26776 Copy
Species: Aequorea victoria and Discosoma sp
Genetic Insert: CAG-TdTomato-2A-H2BGFP-SV40pA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18793381
Proper citation: RRID:Addgene_26771 Copy
Species: Anabaena
Genetic Insert: iLOV
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060199
Proper citation: RRID:Addgene_26770 Copy
Species: Aequorea victoria, Discosoma sp
Genetic Insert: TdTomato-2A-H2BGFP-SV40pA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18793381
Proper citation: RRID:Addgene_26773 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT; PhiC31/attB-attP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20953177
Comments: The pDRAV plasmid series encode a 5' loxP site and a 3' FRT site in all possible orientations for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521
Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906
Proper citation: RRID:Addgene_26749 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060892
Comments: The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal
of Cell Science 112, 4243-4255.
Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.
Proper citation: RRID:Addgene_26741 Copy
Genetic Insert: CFP, mCherry, and cI-YFP
Vector Backbone Description: Backbone Marker:Elowitz Lab; Backbone Size:3700; Vector Backbone:pZS21-cIYFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19029898
Proper citation: RRID:Addgene_26756 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6005; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Comments: There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the LEU2 coding sequence and do not alter function of this plasmid.
Proper citation: RRID:Addgene_26849 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5150; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Comments: There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the LEU2 coding sequence and do not alter function of this plasmid.
Proper citation: RRID:Addgene_26847 Copy
Vector Backbone Description: Backbone Size:4822; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26846 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6488; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26841 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:7587; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26840 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Proper citation: RRID:Addgene_26859 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26856 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26855 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. Myc-tagged STOPS lacking the SOCS box was
subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26857 Copy
Genetic Insert: LoxP-3xPDsRed-LoxP
Vector Backbone Description: Backbone Size:4556; Vector Backbone:pXL-BACII; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: pXL-BACII transformation vector marked by a floxed eye expressing DsRed expression cassette. Cloning sites are EcoRV, SalI, XhoI.
Proper citation: RRID:Addgene_26852 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
