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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
APneg-VAMP1b
 
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RRID:Addgene_168008 VAMP1(T29-K89)-T7PAP Synthetic Spectinomycin PMID:33602850 Additional insert: gIII/luxAB Backbone Marker:David Liu Lab; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:49 0
pMS24: pCascade(PmcCAST)_entry
 
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RRID:Addgene_168157 PmcCAST minimal CRISPR array Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pMS26: pTns(PmcCAST)_ΔTniQ
 
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RRID:Addgene_168159 PmcCAST Tns proteins (TnsAB, TnsC and TnsD) Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pMS21: pHelper(PmcCAST)_entry
 
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1+ mentions
RRID:Addgene_168154 PmcCAST minimal CRISPR array Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 1
pMS23: pHelper(PmcCAST)_entry_ΔTnsD
 
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RRID:Addgene_168156 PmcCAST minimal CRISPR array Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pMS7: pCascade(AvCAST)_entry
 
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RRID:Addgene_168140 AvCAST minimal CRISPR array Anabaena variabilis Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:50 0
pMS6: pHelper(AvCAST)_entry_ΔTnsD
 
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RRID:Addgene_168139 AvCAST minimal CRISPR array Anabaena variabilis Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD
 
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RRID:Addgene_168135 AvCAST minimal CRISPR array (with spacer for AvPSP1) Anabaena variabilis Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pMS4: pHelper(AvCAST)_entry
 
Resource Report
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1+ mentions
RRID:Addgene_168137 AvCAST minimal CRISPR array Anabaena variabilis Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:50 2
pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ
 
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RRID:Addgene_168134 AvCAST minimal CRISPR array (with spacer for AvPSP1) Anabaena variabilis Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:50 0
pMS27: pTns(PmcCAST)_ΔTnsD
 
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RRID:Addgene_168160 PmcCAST Tns proteins (TnsAB and TnsC) Peltigera membranacea cyanobiont 210A Spectinomycin PMID:33770501 Backbone Marker:Millipore Sigma; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:51 0
pAGM1021
 
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RRID:Addgene_169084 BvCYP76AD1 Beta vulgaris Spectinomycin PMID:34177999 Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH41308; Vector Types:Synthetic Biology, MoClo Cloning Vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:56 0
pAGM49981
 
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RRID:Addgene_169087 BvADHα Beta vulgaris Spectinomycin PMID:34177999 Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH41308; Vector Types:Synthetic Biology, MoClo Cloning Vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:09:57 0
pYPQ230-E795L
 
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RRID:Addgene_176890 LbCas12a-E795L Lachnospiraceae bacterium ND2006 Spectinomycin PMID:35174354 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible LbCpf1 entry clone; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:02 0
pYPQ220-Ultra
 
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RRID:Addgene_176889 AsCas12a Acidaminococcus sp. BV3L6 Spectinomycin PMID:35174354 Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible Cas12a entry clone; Bacterial Resistance:Spectinomycin 2026-08-15 01:11:02 0
pLEVI(408)-ColE-iLight-msfGFP
 
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RRID:Addgene_170268 iLight Synthetic Spectinomycin PMID:34162879 Backbone Marker:Y. Yang lab (East China University of Science and Technology, China), https://pubmed.ncbi.nlm.nih.gov/27311594/; Backbone Size:5347; Vector Backbone:pLEVI(408)-ColE; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin To design iLight for expression in bacteria, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V. 2026-08-15 01:10:06 0
Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
 
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RRID:Addgene_170767 M.XbaI Synthetic Spectinomycin PMID:35255734 Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:10:10 0
Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA
 
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RRID:Addgene_170768 M.XhoI Synthetic Spectinomycin PMID:35255734 Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:10:11 0
pGREAT23
 
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RRID:Addgene_170911 E-Luc Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F) Spectinomycin PMID:34520169 Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin c.375C>T silent mutation to remove BbsI site (E-Luc), p.S286Y Red mutant (RedF) 2026-08-15 01:10:12 0
pGREAT24
 
Resource Report
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RRID:Addgene_170912 E-Luc Pyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F) Spectinomycin PMID:34520169 Encodes both E-Luc and Red-F transcriptional units. Insert can be excised by BsaI digestion. Requires pSOUP for replication in agrobacterium . Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv Backbone Marker:Diego Orzaez Lab; Backbone Size:2925; Vector Backbone:pDGB1_omega1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin c.375C>T silent mutation to remove BbsI site (E-Luc), p.S286Y Red mutant (RedF) 2026-08-15 01:10:12 0

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